Nestin Antibody - BSA Free


Western Blot: Nestin Antibody [NBP1-02419] - Densitometric analysis revealed a significant reduction of GFAP and a significant increase of NESTIN in U251MG-Sph compared to U251MG-Adh. Image collected and cropped by more
Immunohistochemistry-Paraffin: Nestin Antibody [NBP1-02419] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 more
Western Blot: Nestin Antibody [NBP1-02419] - Affinity Purified anti-Nestin antibody shows detection of a band ~220 kD corresponding to mouse Nestin (arrowhead). Approximately 30 g of MEF whole cell lysate was separated more
Immunohistochemistry-Paraffin: Nestin Antibody [NBP1-02419] - Staining of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 more

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Nestin Antibody - BSA Free Summary

Recombinant human Nestin corresponding to aa 1484-1500.
1484-1500 of human protein
Neural Stem Cell Marker
Predicted Species
Rabbit (94%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:20000-1:80000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:300
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:500-1:3000
Application Notes
Use in Flow reported in scientific literature (PMID:34021738).
Read Publications using
NBP1-02419 in the following applications:

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 25312433). Rat reactivity reported in scientific literature (PMID; 24012985). Predicted reactivity based on percent identity from BLAST analysis: Hamster (88%).

Packaging, Storage & Formulations

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
0.01% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for Nestin Antibody - BSA Free

  • FLJ21841
  • NES
  • Nestin


Nestin is a Class VI intermediate filament expressed in the developing central nervous system in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Because if it's roles in angiogenisis and tumorgenesis, Nestin antibodies are useful cancer markers.

Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nestin Antibody (NBP1-02419)(8)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 3 applications: FLOW, ICC/IF, WB.

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Showing Publications 1 - 8 of 8.
Publications using NBP1-02419 Applications Species
Bazina F, Brouxhon SM, Kyrkanides S Reprogramming oral epithelial keratinocytes into a pluripotent phenotype for tissue regeneration Clinical and experimental dental research 2021-05-22 [PMID: 34021738] (FLOW, ICC/IF, WB, Human) FLOW, ICC/IF, WB Human
Shan W, Zhou L, Liu L et al. Polycomb group protein Bmi1 is required for the neuronal differentiation of mouse induced pluripotent stem cells Experimental and Therapeutic Medicine 2021-04-14 [PMID: 33936276] (ICC/IF, Mouse) ICC/IF Mouse
Yan J, Goerne T, Zelmer A, et al. The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia Front Cell Dev Biol 2019-11-21 [PMID: 31824945] (ICC/IF, Mouse) ICC/IF Mouse
Berardinelli F, Tanori M et al. G-quadruplex ligand RHPS4 radiosensitizes glioblastoma xenograft in vivo through a differential targeting of bulky differentiated- and stem-cancer cells. J Exp Clin Cancer Res 2019-07-16 [PMID: 31311580] (WB, Human) WB Human
Petersen ED, Zenchak JR, Lossia OV, Hochgeschwender U. Neural Stem Cells Derived Directly from Adipose Tissue. Stem Cells Dev 2018-05-01 [PMID: 29649413]
Cho JH, Patel B, Bonala S et al. Notch transactivates Rheb to maintain the multipotency of TSC-null cells Nat Commun. 2017-11-29 [PMID: 29184052] (ICC/IF, Mouse) ICC/IF Mouse
Oh Hj, Park Je, Park Ej et al. Analysis of cell growth and gene expression of porcine adipose tissue-derived mesenchymal stem cells as nuclear donor cell. Dev. Growth Differ. 2014-10-14 [PMID: 25312433] (ICC/IF, Porcine) ICC/IF Porcine
Ohnishi Y, Iwatsuki K, Shinzawa K et al. Adult olfactory sphere cells are a source of oligodendrocyte and Schwann cell progenitors. Stem Cell Res 2013-11-01 [PMID: 24012985] (Rat) Rat

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Blogs on Nestin.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including ...  Read full blog post.

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Gene Symbol NES