Nestin Antibody - BSA Free

Western Blot: Nestin Antibody [NBP1-02419] - Densitometric analysis revealed a significant reduction of GFAP and a significant increase of NESTIN in U251MG-Sph compared to U251MG-Adh. Image collected and cropped by CiteAb from the following publication (jeccr.biomedcentral.com/articles/10.1186/s13046-019-1293-x), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Nestin Antibody [NBP1-02419] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western Blot: Nestin Antibody [NBP1-02419] - Affinity Purified anti-Nestin antibody shows detection of a band ~220 kD corresponding to mouse Nestin (arrowhead). Approximately 30 g of MEF whole cell lysate was separated by SDS-PAGE using a 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2000 overnight at 4C. The membrane was then washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature.

Immunohistochemistry-Paraffin: Nestin Antibody [NBP1-02419] - Staining of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

Nestin Antibody - BSA Free Summary

• Immunogen: Recombinant human Nestin corresponding to aa 1484-1500.
• Epitope: 1484-1500 of human protein
• Marker: Neural Stem Cell Marker
• Predicted Species: Rabbit (94%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NES
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:80000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:300
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:500-1:3000
• Application Notes: Use in Flow reported in scientific literature (PMID:34021738).

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 25312433). Rat reactivity reported in scientific literature (PMID; 24012985). Predicted reactivity based on percent identity from BLAST analysis: Hamster (88%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nestin Antibody - BSA Free

• FLJ21841
• NES
• Nestin

Background

Nestin is a Class VI intermediate filament expressed in the developing central nervous system in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Because if it's roles in angiogenisis and tumorgenesis, Nestin antibodies are useful cancer markers.

Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nestin Antibody (NBP1-02419)(8)

Publications using NBP1-02419

• Bazina F, Brouxhon SM, Kyrkanides S Reprogramming oral epithelial keratinocytes into a pluripotent phenotype for tissue regeneration Clinical and experimental dental research 2021-05-22 [PMID: 34021738] (FLOW, ICC/IF, WB, Human)
• Shan W, Zhou L, Liu L et al. Polycomb group protein Bmi1 is required for the neuronal differentiation of mouse induced pluripotent stem cells Experimental and Therapeutic Medicine 2021-04-14 [PMID: 33936276] (ICC/IF, Mouse)
• Yan J, Goerne T, Zelmer A, et al. The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia Front Cell Dev Biol 2019-11-21 [PMID: 31824945] (ICC/IF, Mouse)
• Berardinelli F, Tanori M et al. G-quadruplex ligand RHPS4 radiosensitizes glioblastoma xenograft in vivo through a differential targeting of bulky differentiated- and stem-cancer cells. J Exp Clin Cancer Res 2019-07-16 [PMID: 31311580] (WB, Human)
• Petersen ED, Zenchak JR, Lossia OV, Hochgeschwender U. Neural Stem Cells Derived Directly from Adipose Tissue. Stem Cells Dev 2018-05-01 [PMID: 29649413]
• Cho JH, Patel B, Bonala S et al. Notch transactivates Rheb to maintain the multipotency of TSC-null cells Nat Commun. 2017-11-29 [PMID: 29184052] (ICC/IF, Mouse)
• Oh Hj, Park Je, Park Ej et al. Analysis of cell growth and gene expression of porcine adipose tissue-derived mesenchymal stem cells as nuclear donor cell. Dev. Growth Differ. 2014-10-14 [PMID: 25312433] (ICC/IF, Porcine)
• Ohnishi Y, Iwatsuki K, Shinzawa K et al. Adult olfactory sphere cells are a source of oligodendrocyte and Schwann cell progenitors. Stem Cell Res 2013-11-01 [PMID: 24012985] (Rat)

Bioinformatics

• Gene Symbol: NES
• Entrez:
  • 10763(Human)
  • 18008(Mouse)
  • 25491(Rat)
• Uniprot:
  • P48681()