Nestin Antibody [HRP]

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Product Details

Summary
Reactivity Hu, Ca, Mu(-), Rb(-), Rt(-)Species Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Nestin Antibody [HRP] Summary

Immunogen
Recombinant human Nestin protein (corresponding to residues 1464-1614). [UniProt# P48681]
Localization
Cytoplasmic
Marker
Neural Stem Cell Marker
Specificity
This is specific for human nestin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NES
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This Nestin antibody may be used in Immunocytochemistry/Immunofluorescence and Western blot where a band is seen at ~220-240 kDa. Another band may be seen ~110-115 kDa. Other applications have not been tested. Though this production has not been tested, past productions have been useful for Immunohistochemistry and Immunoprecipitation. The investigator should determine the optimal working dilution for a specific application.
Readout System

Reactivity Notes

Human. Canine reactivity reported in scientific literature (PMID: 25088159) Does not cross-react with rodent.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Nestin Antibody [HRP]

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nestin Antibody (NB300-265H) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Nestin Antibody (NB300-265H) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB300-265B
DyLight 488 Labeled NB300-265G
DyLight 550 Labeled NB300-265R
DyLight 650 Labeled NB300-265C

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Bioinformatics Tool for Nestin Antibody (NB300-265H)

Discover related pathways, diseases and genes to Nestin Antibody (NB300-265H). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

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Research Areas for Nestin Antibody (NB300-265H)

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Blogs on Nestin.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including...  Read full blog post.

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Bioinformatics

Gene Symbol NES
Entrez