Nestin Antibody

Images

 
Western Blot: Nestin Antibody [NB300-265] - Detection of nestin protein in human fetal temporal lobe tissue lysates (30 ug). Lane 1: 0.5 ug/ml NB 300-265: lane 2: 2 ug/ml NB 300-265. ECL: 1 second exposure.
Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB300-265] - The Nestin antibody was tested in NTERA2 cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) ...read more

Product Details

Summary
Reactivity Hu, Ca, Mu(-), Rb(-), Rt(-)Species Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Nestin Antibody Summary

Immunogen
Recombinant human Nestin protein (corresponding to residues 1464-1614). [UniProt# P48681]
Localization
Cytoplasmic
Marker
Neural Stem Cell Marker
Specificity
This is specific for human nestin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NES
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
Application Notes
This Nestin antibody may be used in Immunocytochemistry/Immunofluorescence and Western blot where a band is seen at ~220-240 kDa. Another band may be seen ~110-115 kDa. Other applications have not been tested. Though this production has not been tested, past productions have been useful for Immunohistochemistry and Immunoprecipitation. The investigator should determine the optimal working dilution for a specific application.
Positive Control
Brain Temporal Lobe Lysate (NB820-59476)
Publications
Read Publications using
NB300-265 in the following applications:

  • WB
    2 publications

Reactivity Notes

Human. Canine reactivity reported in scientific literature (PMID: 25088159) Does not cross-react with rodent.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nestin Antibody

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-141
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-223
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB120-15200
Species: Hu, Mu, Sh
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-213
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-Fr
NB100-2379
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
NB120-16518
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB2018
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, ICC, ICFlow
NB100-79802
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB110-58870
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF
248-BD/CF
Species: Hu, Mu, Rt, Ca, Eq
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF3795
Species: Hu
Applications: WB, IP
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF

Publications for Nestin Antibody (NB300-265)(2)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
All Applications
Filter By Species
Human
(1)
Canine
(1)
All Species

Reviews for Nestin Antibody (NB300-265) (0)

There are no reviews for Nestin Antibody (NB300-265). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for Nestin Antibody (NB300-265): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nestin Antibody (NB300-265) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-265AF405
Alexa Fluor 488 Labeled NB300-265AF488
Alexa Fluor 647 Labeled NB300-265AF647
Alexa Fluor 700 Labeled NB300-265AF700
Biotin Labeled NB300-265B
DyLight 405 Labeled NB300-265V
DyLight 488 Labeled NB300-265G
DyLight 550 Labeled NB300-265R
DyLight 650 Labeled NB300-265C
FITC Labeled NB300-265F
HRP Labeled NB300-265H

Additional Nestin Products

Bioinformatics Tool for Nestin Antibody (NB300-265)

Discover related pathways, diseases and genes to Nestin Antibody (NB300-265). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Nestin Antibody (NB300-265)

Discover more about diseases related to Nestin Antibody (NB300-265).
 

Pathways for Nestin Antibody (NB300-265)

View related products by pathway.

PTMs for Nestin Antibody (NB300-265)

Learn more about PTMs related to Nestin Antibody (NB300-265).
 

Research Areas for Nestin Antibody (NB300-265)

Find related products by research area.

Blogs on Nestin.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Nestin Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NES
Entrez
Uniprot