Nestin Antibody


Western Blot: Nestin Antibody [NB300-265] - Detection of nestin protein in human fetal temporal lobe tissue lysates (30 ug). Lane 1: 0.5 ug/ml NB 300-265: lane 2: 2 ug/ml NB 300-265. ECL: 1 second exposure.
Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB300-265] - The Nestin antibody was tested in NTERA2 cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) more

Product Details

Reactivity Hu, Ca, Mu(-), Rb(-), Rt(-)Species Glossary
Applications WB, ICC/IF
1 mg/ml

Nestin Antibody Summary

Recombinant human Nestin protein (corresponding to residues 1464-1614). [UniProt# P48681]
Neural Stem Cell Marker
This is specific for human nestin.
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:500-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
Application Notes
This Nestin antibody may be used in Immunocytochemistry/Immunofluorescence and Western blot where a band is seen at ~220-240 kDa. Another band may be seen ~110-115 kDa. Other applications have not been tested. Though this production has not been tested, past productions have been useful for Immunohistochemistry and Immunoprecipitation. The investigator should determine the optimal working dilution for a specific application.
Read Publications using
NB300-265 in the following applications:

  • WB
    2 publications

Reactivity Notes

Human. Canine reactivity reported in scientific literature (PMID: 25088159) Does not cross-react with rodent.

Alternate Names for Nestin Antibody

  • FLJ21841
  • NES
  • nestin


Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF, IHC-P, IP, PLA
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF
Species: Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, IP
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF

Publications for Nestin Antibody (NB300-265)(2)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 1 application: WB.

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Product General Protocols

View specific protocols for Nestin Antibody (NB300-265): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nestin Antibody (NB300-265) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB300-265AF405
Alexa Fluor (R) 488 Labeled NB300-265AF488
Alexa Fluor (R) 647 Labeled NB300-265AF647
Alexa Fluor (R) 700 Labeled NB300-265AF700
Biotin Labeled NB300-265B
DyLight 405 Labeled NB300-265V
DyLight 488 Labeled NB300-265G
DyLight 550 Labeled NB300-265R
DyLight 650 Labeled NB300-265C
FITC Labeled NB300-265F
HRP Labeled NB300-265H

Additional Nestin Antibody Products

Nestin NB300-265

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Blogs on Nestin.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol NES

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