Nestin Antibody

Images

 
Immunohistochemistry: Nestin Antibody [NB100-1604] - A tissue section through an e 13 mouse brain showing Nestin (green staining) in neural stem cells of the ventricular zone of the CNS. DAPI (blue staining) allows ...read more
Immunohistochemistry-Frozen: Nestin Antibody [NB100-1604] - Analysis of Nestin in frozen mouse tissue using anti-Nestin antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

Nestin Antibody Summary

Immunogen
Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the mouse nestin gene product (NP_057910).
Localization
Cytoplasmic
Marker
Neural Stem Cell Marker
Specificity
Nestin
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
NES
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:20000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:10000-1:20000
  • Immunohistochemistry 1:10000-1:20000
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:10000-1:20000
Application Notes
Use in Immunohistochemistry on frozen sections was reported in a scientific publication (PMID: 23741377). See publications for use in ICC/IF.
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-1604 in the following applications:

Publications
Read Publications using
NB100-1604 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:19481056).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Sodium PBS (0.9% isotonic, w/v, pH 7.2)
Preservative
0.02% Sodium Azide
Concentration
0.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nestin Antibody

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nestin Antibody (NB100-1604)(29)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(11)
IHC
(9)
IHC-Fr
(3)
IHC-P
(2)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(18)
Rat
(1)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB100-1604 Applications Species
Dutta D, Hickey K, Salifu M et al. Spatiotemporal presentation of exogenous SDF-1 with PLGA nanoparticles modulates SDF-1/CXCR4 signaling axis in the rodent cortex Biomater Sci 2017 Jul 25 [PMID: 28703822] (Mouse) Mouse
Yadav VN, Zamler D, Baker GJ et al. CXCR4 increases in-vivo glioma perivascular invasion, and reduces radiation induced apoptosis: A genetic knockdown study. Oncotarget. Nov 11 2016 [PMID: 27863376] (IHC, Mouse) IHC Mouse
Borgs L, Peyre E, Alix P et al. Dopaminergic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects Sci Rep Sep 19 2016 [PMID: 27640816] (ICC/IF, Human) ICC/IF Human
Vasilopoulou E, Joannou MK, Lindenmeyer MT et al. Loss of endogenous thymosin beta 4 accelerates glomerular disease. Kidney Int. Sep 26 2016 [PMID: 27575556] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Wang Y, Xu P, Qiu L et al. CXCR7 Participates in CXCL12-mediated Cell Cycle and Proliferation Regulation in Mouse Neural Progenitor Cells. Curr. Mol. Med. Aug 30 2016 [PMID: 27573194] (ICC/IF, Mouse) ICC/IF Mouse
Fantetti KN, Gray EL, Ganesan P et al. Interferon gamma protects neonatal neural stem/progenitor cells during measles virus infection of the brain. J Neuroinflammation. May 14 2016 [PMID: 27178303] (IHC-Fr, Mouse) IHC-Fr Mouse
Koutsoudaki PN, Papastefanaki F, Stamatakis A et al. Neural stem/progenitor cells differentiate into oligodendrocytes, reduce inflammation, and ameliorate learning deficits after transplantation in a mouse model of traumatic brain injury. Glia. 2015 Dec 29 [PMID: 26712314] (IHC-Fr, Mouse) IHC-Fr Mouse
Neirinckx V, Agirman G, Coste C et al. Adult bone marrow mesenchymal and neural crest stem cells are chemoattractive and accelerate motor recovery in a mouse model of spinal cord injury. Stem Cell Res Ther 2015 [PMID: 26530515] (ICC/IF, Mouse) ICC/IF Mouse
Kim KA, Kang SW, Ahn HR et al. The Leaves of Persimmon (Diospyros kaki Thunb.) Ameliorate N-methyl-N-nitrosourea (MNU)-Induced Retinal Degeneration in Mice. J. Agric. Food Chem. 2015 Aug 11 [PMID: 26260943] (WB, IHC-P, Mouse) WB, IHC-P Mouse
Braun GS, Nagayama Y, Maruta Y et al. IL-6 Trans-Signaling Drives Murine Crescentic GN. J. Am. Soc. Nephrol. Jan 1 2016 [PMID: 26041841] (IHC, Mouse) IHC Mouse
Show All 29 Publications.

Reviews for Nestin Antibody (NB100-1604) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 1 application: IF.

Reviews using NB100-1604:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
 Nestin NB100-1604
Enlarge
reviewed by:
Anonymous
Mouse 01/09/2015
View

Summary

SpeciesMouse
FileView PDF
 
reviewed by:
Anonymous
IF Mouse 12/12/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23741377
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23741377

Details

Detection NotesSee PMID 23741377

Comments

CommentsPublished in PMID: 23741377

Product General Protocols

View specific protocols for Nestin Antibody (NB100-1604): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nestin Antibody (NB100-1604). (Showing 1 - 1 of 1 FAQs).

  1. Is this antibody from serum or egg? Is it IgG or IgY antibody? Which secondary antibody we should use?
    • This antibody was purified from egg yolks. This antibody is isotype IgY. You should definitely use IgY secondary with this antibody.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Nestin Antibody (NB100-1604)

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Blogs on Nestin.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
01/09/2015
Application:
Species: Mouse

 
Anonymous
12/12/2014
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol NES
Entrez
Uniprot