Nestin Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Photomicrograph of 3T3 cells in culture. Nestin immunoreactivity (RED staining); GREEN is beta-Tubulin 3 staining using a rabbit antibody; BLUE is ...read more
Immunohistochemistry-Paraffin: Nestin Antibody [NB100-1604] - Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse ...read more
Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Immunocytochemistry showing nestin immunoreactivity (GREEN) in A7r5 neuroblastoma cells in culture. BLUE is DAPI nuclear staining.
Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Characterization of MSCmix and NCSCmix isolated from the bone marrow of adult Wnt1-CRE/R26R-LacZ mice. NCSCmix express Nestin (green), Sox2 (red) ...read more
Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Characterization of MSCmix and NCSCmix isolated from the bone marrow of adult Wnt1-CRE/R26R-LacZ mice. MSCmix are adherent fibroblast-like cells, ...read more
Immunohistochemistry-Frozen: Nestin Antibody [NB100-1604] - Analysis of Nestin in frozen mouse tissue using anti-Nestin antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

Nestin Antibody Summary

Immunogen
Chickens were immunized with three synthetic peptide/keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of mouse Nestin (NM_NP_057910), but are shared with the rat (NM_012987, NCBI) protein sequence.
Localization
Cytoplasmic
Marker
Neural Stem Cell Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
NES
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:20000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10000-1:20000
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:10000-1:20000
Application Notes
Each of the three antibodies were analyzed by immunohistochemistry (1:20000) using fluorescein-labeled goat anti-chicken IgY (1:500) as the secondary reagent. Use in Immunohistochemistry on frozen sections was reported in a scientific publication (PMID: 23741377). See publications for use in ICC/IF. For WB, use only nitrocellulose membranes, and not PVDF membranes.
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 5
using
NB100-1604 in the following applications:

Publications
Read Publications using
NB100-1604 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:19481056). Baboon reactivity reported in scientific literature (PMID: 30657788 ). Monkey reactivity reported in scientific literature (PMID: 30657788).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Sodium PBS (0.9% isotonic, w/v, pH 7.2)
Preservative
0.02% Sodium Azide
Concentration
0.3 mg/ml
Purity
Immunogen affinity purified

Notes

Purification Notes
After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 ug/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter sterilized.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Alternate Names for Nestin Antibody

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a Class VI intermediate filament expressed in the developing central nervous system in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Because if it's roles in angiogenisis and tumorgenesis, Nestin antibodies are useful cancer markers.

Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nestin Antibody (NB100-1604)(40)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Monkey.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(17)
IHC
(12)
IHC-Fr
(4)
IHC-P
(2)
WB
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(26)
Rat
(1)
Monkey
(1)
All Species
Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using NB100-1604 Applications Species
Xu D, Zhao J RADA16-I Hydrogel-Released CXCL12 Stably Promotes Stem Cell Migration J Tissue Sci Eng Feb 18 2019 (ICC/IF, Mouse) ICC/IF Mouse
Kim S, Kim N, Park S et al. Tanycytic TSPO inhibition induces lipophagy to regulate lipid metabolism and improve energy balance Autophagy Aug 30 2019 [PMID: 31469345] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Fu CH, Iascone DM, Petrof I et al. Early Seizure Activity Accelerates Depletion of Hippocampal Neural Stem Cells and Impairs Spatial Discrimination in an Alzheimer's Disease Model Cell Rep Jun 25 2019 [PMID: 31242408]
Zhu, S;Liu, W;Ding, HF;Cui, H;Yang, L; BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation Exp Ther Med May 1 2019 [PMID: 31007733] (ICC/IF, Mouse) ICC/IF Mouse
Gurung S, Reuter N, Preno A et al. Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon PLoS Pathog. Jan 1 2019 [PMID: 30657788] (IHC-Fr, Monkey) IHC-Fr Monkey
Yeon JY, Hwang JY, Lee HW et al. Optimized Clump Culture Methods for Adult Human Multipotent Neural Cells. Int J Mol Sci. Oct 29 2018 [PMID: 30380605] (ICC/IF, Human) ICC/IF Human
MoaYedi Y, Duenas-Bianchi LF, Lumpkin EA. Somatosensory innervation of the oral mucosa of adult and aging mice. Sci Rep. Jul 2 2018 [PMID: 29967482] (ICC/IF, Mouse) ICC/IF Mouse
Choi CI, Yoon H, Drucker KL et al. The Thrombin Receptor Restricts Subventricular Zone Neural Stem Cell Expansion and Differentiation Sci Rep Jun 19 2018 [PMID: 29921916] (ICC/IF, Mouse) ICC/IF Mouse
Morton MC, Neckles VN, Seluzicki CM et al. Neonatal Subventricular Zone Neural Stem Cells Release Extracellular Vesicles that Act as a Microglial Morphogen. Cell Rep Apr 3 2018 [PMID: 29617675] (IHC, Mouse) IHC Mouse
Kirschen GW, Kery R, Liu H et al. Genetic dissection of the neuro-glio-vascular machinery in the adult brain Mol Brain 2018 Jan 15 [PMID: 29335006] (IHC, Mouse) IHC Mouse
Show All 40 Publications.

Reviews for Nestin Antibody (NB100-1604) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB100-1604:
Filter by Applications
ICC
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC Mouse 05/01/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedbEnd.3 mouse endothelioma cell line
SpeciesMouse
LotNES69695
 Nestin NB100-1604
Enlarge
reviewed by:
Anonymous
Mouse 01/09/2015
View

Summary

SpeciesMouse
FileView PDF
 
reviewed by:
Anonymous
IF Mouse 12/12/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23741377
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23741377

Details

Detection NotesSee PMID 23741377

Comments

CommentsPublished in PMID: 23741377

Product General Protocols

View specific protocols for Nestin Antibody (NB100-1604): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nestin Antibody (NB100-1604). (Showing 1 - 1 of 1 FAQs).

  1. Is this antibody from serum or egg? Is it IgG or IgY antibody? Which secondary antibody we should use?
    • This antibody was purified from egg yolks. This antibody is isotype IgY. You should definitely use IgY secondary with this antibody.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Blogs on Nestin.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

 
Anonymous
05/01/2019
Application: ICC
Species: Mouse

Anonymous
01/09/2015
Application:
Species: Mouse

 
Anonymous
12/12/2014
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol NES
Entrez
Uniprot