Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Photomicrograph of 3T3 cells in culture. Nestin immunoreactivity (RED staining); GREEN is beta-Tubulin 3 staining using a rabbit antibody; BLUE is ...read more
Immunohistochemistry-Paraffin: Nestin Antibody [NB100-1604] - Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse ...read more
Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Immunocytochemistry showing nestin immunoreactivity (GREEN) in A7r5 neuroblastoma cells in culture. BLUE is DAPI nuclear staining.
Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Characterization of MSCmix and NCSCmix isolated from the bone marrow of adult Wnt1-CRE/R26R-LacZ mice. NCSCmix express Nestin (green), Sox2 (red) ...read more
Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Characterization of MSCmix and NCSCmix isolated from the bone marrow of adult Wnt1-CRE/R26R-LacZ mice. MSCmix are adherent fibroblast-like cells, ...read more
Immunohistochemistry-Frozen: Nestin Antibody [NB100-1604] - Analysis of Nestin in frozen mouse tissue using anti-Nestin antibody. Image from verified customer review.
Chickens were immunized with three synthetic peptide/keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of mouse Nestin (NM_NP_057910), but are shared with the rat (NM_012987, NCBI) protein sequence.
Neural Stem Cell Marker
Immunogen affinity purified
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Each of the three antibodies were analyzed by immunohistochemistry (1:20000) using fluorescein-labeled goat anti-chicken IgY (1:500) as the secondary reagent. Use in Immunohistochemistry on frozen sections was reported in a scientific publication (PMID: 23741377). See publications for use in ICC/IF. For WB, use only nitrocellulose membranes, and not PVDF membranes.
250 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 3 Reviews rated 5 using NB100-1604 in the following applications:
Rat reactivity reported in scientific literature (PMID:19481056). Baboon reactivity reported in scientific literature (PMID: 30657788 ). Monkey reactivity reported in scientific literature (PMID: 30657788).
Packaging, Storage & Formulations
Store at 4C in the dark.
10mM Sodium PBS (0.9% isotonic, w/v, pH 7.2)
0.02% Sodium Azide
Immunogen affinity purified
Purification Notes After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 ug/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter sterilized.
Storage Notes Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Alternate Names for Nestin Antibody
Nestin is a Class VI intermediate filament expressed in the developing central nervous system in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Because if it's roles in angiogenisis and tumorgenesis, Nestin antibodies are useful cancer markers.
Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.