Nestin Antibody [Alexa Fluor® 488]

• Reactivity: Rt
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Nestin Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant rat Nestin
  Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
  Accession # EDM00749
• Specificity: Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nestin Antibody [Alexa Fluor® 488]

• FLJ21841
• NES
• Nestin

Background

Nestin is a 240 kDa class VI intermediate filament protein that was first identified in neuroepithelial stem cells (1, 2). It has been used extensively as a marker to identify central nervous system (CNS) stem cell populations. In addition, Nestin has been found to be a marker for multi-lineage progenitor cells. Over the region used for immunization, rat Nestin shares 76% amino acid sequence homology with the mouse protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.