Nesprin 2 Antibody

Western Blot: Nesprin 2 Antibody [NBP2-42886] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nesprin 2 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886] - Rat brain. Nesprin 2 stained by Nesprin 2 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Frozen: Nesprin 2 Antibody [NBP2-42886] - Frozen sectioned adult mouse retina. Green: Nesprin 2 protein stained by Nesprin 2 antibody [C3], C-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:250. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886] - Mouse kidney. Nesprin 2 stained by Nesprin 2 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody [NBP2-42886] - Syne2 antibody [C3], C-term detects Syne2 protein at nucleus by immunofluorescent analysis.Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Syne2 protein stained by Syne2 antibody [C3], C-term (NBP2-42886) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody [NBP2-42886] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nesprin 2 stained by Nesprin 2 antibody [C3], C-term diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Nesprin 2 Antibody Summary

• Description: Novus Biologicals Rabbit Nesprin 2 Antibody (NBP2-42886) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of mouse Nesprin 2. The exact sequence is proprietary.
• Predicted Species: Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYNE2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 783 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Nesprin 2 Antibody

• DKFZP434H2235
• DKFZp686E01115
• DKFZp686H1931
• EDMD5
• FLJ46790
• KIAA1011FLJ11014
• nesprin 2
• Nesprin-2
• NUAFLJ43727
• NUANCEFLJ45710
• Nuclear envelope spectrin repeat protein 2
• Nucleus and actin connecting element protein
• polytrophin
• Protein NUANCE
• spectrin repeat containing, nuclear envelope 2
• Synaptic nuclear envelope protein 2
• synaptic nuclei expressed gene 2
• SYNE-2
• TROPH

Background

SYNE2 - spectrin repeat containing, nuclear envelope 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SYNE2