| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84190-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38620-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59944-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59945-0.025mg | |||
![Western Blot: Nesprin 2 Antibody [NBP2-42886] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nesprin 2 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Western-Blot-NBP2-42886-img0006.jpg "Western Blot: Nesprin 2 Antibody [NBP2-42886] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nesprin 2 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886] - Rat brain. Nesprin 2 stained by Nesprin 2 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-42886-img0008.jpg)
![Immunohistochemistry-Frozen: Nesprin 2 Antibody [NBP2-42886] - Frozen sectioned adult mouse retina. Green: Nesprin 2 protein stained by Nesprin 2 antibody [C3], C-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Frozen-NBP2-42886-img0004.jpg)
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886] - Mouse kidney. Nesprin 2 stained by Nesprin 2 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-42886-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody [NBP2-42886] - Syne2 antibody [C3], C-term detects Syne2 protein at nucleus by immunofluorescent analysis.Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Syne2 protein stained by Syne2 antibody [C3], C-term (NBP2-42886) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-42886_rabbit-polyclonal-nesprin-2-antibody-91020242011444.jpg)
![Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody [NBP2-42886] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nesprin 2 stained by Nesprin 2 antibody [C3], C-term diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.](http://images.novusbio.com/fullsize/antibody/nbp2-42886_rabbit-polyclonal-nesprin-2-antibody-immunocytochemistry-immunofluorescence-273202514336..png)
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP1-84190] - Staining of human cerebral cortex, kidney, skin and testis using Anti-SYNE2 antibody NBP1-84190 (A) shows similar protein distribution across tissues to independent antibody NBP2-38620 (B).](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-84190-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP1-84190] - Staining of human cerebral cortex, kidney, skin and testis using Anti-SYNE2 antibody NBP1-84190 (A) shows similar protein distribution across tissues to independent antibody NBP2-38620 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP1-84190] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-84190-img0015.jpg)
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP1-84190] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-84190-img0011.jpg)
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP1-84190] - Staining of human kidney shows moderate to strong positivity in nuclear membrane in cells in tubules.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-84190-img0013.jpg)
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP1-84190] - Staining of human skin shows moderate positivity in nuclear membrane in squamous epithelial cells.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-84190-img0014.jpg)
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human cerebral cortex, kidney, skin and testis using Anti-SYNE2 antibody NBP2-38620 (A) shows similar protein distribution across tissues to independent antibody NBP1-84190 (B).](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38620-img0010.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human cerebral cortex, kidney, skin and testis using Anti-SYNE2 antibody NBP2-38620 (A) shows similar protein distribution across tissues to independent antibody NBP1-84190 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Nesprin 2 Antibody [NBP2-38620] - Immunofluorescent staining of human cell line MCF7 shows localization to nuclear membrane & intermediate filaments.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38620-img0003.jpg)
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38620-img0013.jpg)
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38620-img0009.jpg)
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human kidney shows moderate to strong positivity in nuclear membrane in cells in tubules.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38620-img0011.jpg)
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human skin shows moderate positivity in nuclear membrane in squamous epithelial cells.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38620-img0012.jpg)
![Western Blot: Nesprin 2 Antibody (6C11) [NBP2-59944] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform contains the ~180 residue Nesp2 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from three independent anti-Nesp2 hybridoma clones (6C11, 10H8 and 5C3)](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-6C11-Western-Blot-NBP2-59944-img0001.jpg "Western Blot: Nesprin 2 Antibody (6C11) [NBP2-59944] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform contains the ~180 residue Nesp2 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from three independent anti-Nesp2 hybridoma clones (6C11, 10H8 and 5C3)")
![Immunocytochemistry/Immunofluorescence: Nesprin 2 Antibody (6C11) [NBP2-59944] - Immunofluorescence microscopy of HeLa cells. The antibodies can be used on either formaldehyde- or methanol-fixed cells.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-6C11-Immunocytochemistry-Immunofluorescence-NBP2-59944-img0002.jpg)
![Western Blot: Nesprin 2 Antibody (10H8) [NBP2-59945] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform contains the ~180 residue Nesp2 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from three independent anti-Nesp2 hybridoma clones (6C11, 10H8 and 5C3)](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-10H8-Western-Blot-NBP2-59945-img0001.jpg "Western Blot: Nesprin 2 Antibody (10H8) [NBP2-59945] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform contains the ~180 residue Nesp2 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from three independent anti-Nesp2 hybridoma clones (6C11, 10H8 and 5C3)")
![Immunocytochemistry/Immunofluorescence: Nesprin 2 Antibody (10H8) [NBP2-59945] - Staining in HeLa cells labeled with either 6C11 or 10H8 monoclonal antibodies raised against human Nesp2. The antibodies can be used on either formaldehyde- or methanol-fixed cells.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-10H8-Immunocytochemistry-Immunofluorescence-NBP2-59945-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Nesprin 2 Antibody (5E5) [H00023224-M01] - Analysis of monoclonal antibody to SYNE2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-5E5-Immunocytochemistry-Immunofluorescence-H00023224-M01-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nesprin 2 Antibody (5E5) [H00023224-M01] - Analysis of monoclonal antibody to SYNE2 on HeLa cell . Antibody concentration 10 ug/ml.")
![Sandwich ELISA: Nesprin 2 Antibody (5E5) [H00023224-M01] - Detection limit for recombinant GST tagged SYNE2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-5E5-Sandwich-ELISA-H00023224-M01-img0004.jpg)
![Immunoprecipitation:Nesprin 2 AntibodyNBP3-29852] -Detection of human SYNE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SYNE2 antibody NBP3-29852 (lot NBP3-29852-1) used for IP at 6 µg per reaction. SYNE2 was also immunoprecipitated by rabbit anti-SYNE2.. For blotting immunoprecipitated SYNE2, NBP3-29852 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29852_rabbit-nesprin-2-pab-301220241924714.jpg "Immunoprecipitation:Nesprin 2 AntibodyNBP3-29852] -Detection of human SYNE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SYNE2 antibody NBP3-29852 (lot NBP3-29852-1) used for IP at 6 µg per reaction. SYNE2 was also immunoprecipitated by rabbit anti-SYNE2.. For blotting immunoprecipitated SYNE2, NBP3-29852 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:Nesprin 2 AntibodyNBP3-30126] -Detection of human SYNE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SYNE2 antibody NBP3-30126 (lot NBP3-30126-1) used for IP at 6 µg per reaction. SYNE2 was also immunoprecipitated by rabbit anti-SYNE2.. For blotting immunoprecipitated SYNE2, NBP3-30126 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30126_rabbit-nesprin-2-pab-301220241826128.jpg "Immunoprecipitation:Nesprin 2 AntibodyNBP3-30126] -Detection of human SYNE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SYNE2 antibody NBP3-30126 (lot NBP3-30126-1) used for IP at 6 µg per reaction. SYNE2 was also immunoprecipitated by rabbit anti-SYNE2.. For blotting immunoprecipitated SYNE2, NBP3-30126 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![ELISA: Human Nesprin 2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39113] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39113_human-nesprin-2-ready-to-use-elisa-kit-colorimetric-211020241529192.png "ELISA: Human Nesprin 2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39113] - Standard Curve Reference")
![ELISA: Human Nesprin 2 ELISA Kit (Colorimetric) [NBP3-39089] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39089_human-nesprin-2-elisa-kit-colorimetric-2110202415273844.png "ELISA: Human Nesprin 2 ELISA Kit (Colorimetric) [NBP3-39089] - Standard Curve Reference")