Meckelin Antibody

Western Blot: Meckelin Antibody [NBP1-06590] - Immunodetection of Meckelin (120kDa) in whole cell extracts of HEK293 cells.

Immunohistochemistry-Paraffin: Meckelin Antibody [NBP1-06590] - Staining of kidney tissue section with Meckelin Antibody (NBP1-06590).

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Meckelin Antibody Summary

• Immunogen: Synthetic peptide derived from Cter domain of human Meckelin protein
• Specificity: Reacts specifically with human 111 kDa protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMEM67
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:1000
• Application Notes: IHC-Frozen, ICC/IF applications reported in scientific literature (PMID: 23516626)
• Control Peptide:

  Meckelin Protein (NBP1-06590PEP)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23516626). Predicted to cross react with many other species like Guinea pig, Sheep, cat, dog, Porcine due to sequence identity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for Meckelin Antibody

• meckel syndrome type 3 protein
• Meckelin
• MGC26979
• TNEM67
• transmembrane protein 67

Background

Meckelin localizes to the primary cilium and to the plasma membrane. It functions in centriole migration to the apical membrane and formation of the primary cilium. Defects in the gene are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Meckelin Antibody (NBP1-06590)(2)

Publications using NBP1-06590

• Stayner C, Poole C A et al. An ovine hepatorenal fibrocystic model of a Meckel-like syndrome associated with dysmorphic primary cilia and TMEM67 mutations. Sci Rep Sep 5 2017 [PMID: 28487520] (IHC, Human)
• Tiwari S, Hudson S, Gattone VH 2nd et al. Meckelin 3 Is Necessary for Photoreceptor Outer Segment Development in Rat Meckel Syndrome PLoS One 2013 [PMID: 23516626] (IHC-Fr, ICC/IF, Rat)

Bioinformatics

• Gene Symbol: TMEM67
• Entrez:
  • 91147(Human)
  • 329795(Mouse)
  • 313067(Rat)
• Uniprot:
  • Q5HYA8(Human)