Meckelin Antibody

Western Blot: Meckelin Antibody [NBP1-06590] - Immunodetection of Meckelin (120kDa) in whole cell extracts of HEK293 cells.

Immunohistochemistry: Meckelin Antibody [NBP1-06590] - Confocal maximum projection of acetylated alpha-tubulin (red) and meckelin (green) labelling in sections from unaffected and affected kidneys. In unaffected kidneys, meckelin was present within the cytoplasm of the epithelial cells and strongly localised to the base of the cilium. In contrast, meckelin was distributed along the length of the ciliary axonemes of affected cyst-lining cells. Meckelin distribution within the cytoplasm of cyst lining cells was similar to observations in unaffected epithelia. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28487520/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Meckelin Antibody [NBP1-06590] - Staining of kidney tissue section with Meckelin Antibody (NBP1-06590).

Immunocytochemistry/ Immunofluorescence: Meckelin Antibody [NBP1-06590] - Meckelin expression in unaffected & affected lamb kidney epithelial cells & primary cilia. (a, left) Immunoblot showing expression of meckelin (~100 KDa) in cultured epithelial cells from an unaffected lamb kidney, & an affected lamb kidney. beta -actin indicates equivalent protein loading in each lane. (a, right) Quantification of the meckelin band in the immunoblot, normalised to the loading control, shows no significant difference in relative protein expression. (b) Confocal maximum projection of acetylated alpha -tubulin (red) & meckelin (green) labelling in sections from unaffected & affected kidneys. In unaffected kidneys, meckelin was present within the cytoplasm of the epithelial cells & strongly localised to the base of the cilium. In contrast, meckelin was distributed along the length of the ciliary axonemes of affected cyst-lining cells. Meckelin distribution within the cytoplasm of cyst lining cells was similar to observations in unaffected epithelia (c) Acetylated alpha -tubulin (red) & meckelin (green) labelling on putative ciliary fragments located in the cyst lumen of affected kidneys (yellow indicates co-localisation in the merged image). Numerous smaller particles contained in the cyst luminal contents were also labeled with meckelin, but were not labeled with acetylated alpha -tubulin. Scale bars = 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28487520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Meckelin Antibody Summary

• Immunogen: Synthetic peptide derived from Cter domain of human Meckelin protein
• Specificity: Reacts specifically with human 111 kDa protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMEM67
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID: 23516626)
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen Reported in scientific literature (PMID: 23516626)
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:1000
• Control Peptide:

  Meckelin Protein (NBP1-06590PEP)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23516626). Predicted to cross react with many other species like Guinea pig, Sheep, cat, dog, Porcine due to sequence identity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for Meckelin Antibody

• meckel syndrome type 3 protein
• Meckelin
• MGC26979
• TNEM67
• transmembrane protein 67

Background

Meckelin localizes to the primary cilium and to the plasma membrane. It functions in centriole migration to the apical membrane and formation of the primary cilium. Defects in the gene are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Meckelin Antibody (NBP1-06590)(2)

Publications using NBP1-06590

• Stayner C, Poole C A et al. An ovine hepatorenal fibrocystic model of a Meckel-like syndrome associated with dysmorphic primary cilia and TMEM67 mutations. Sci Rep 2017-09-05 [PMID: 28487520] (IF/IHC, Human)
• Tiwari S, Hudson S, Gattone VH 2nd et al. Meckelin 3 Is Necessary for Photoreceptor Outer Segment Development in Rat Meckel Syndrome PLoS One 2013-01-01 [PMID: 23516626] (ICC/IF, IHC-Fr, Rat)

Bioinformatics

• Gene Symbol: TMEM67
• Entrez:
  • 91147(Human)
  • 329795(Mouse)
  • 313067(Rat)
• Uniprot:
  • Q5HYA8(Human)