![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human cerebral cortex, kidney, skin and testis using Anti-SYNE2 antibody NBP2-38620 (A) shows similar protein distribution across tissues to independent antibody NBP1-84190 (B).](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38620-img0010.jpg "Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human cerebral cortex, kidney, skin and testis using Anti-SYNE2 antibody NBP2-38620 (A) shows similar protein distribution across tissues to independent antibody NBP1-84190 (B).")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: GTTPPIEADTLDSSDAQGGLEPRVEKTRPEPTEVLHACKTQVAELELWLQQANVAVEPETLNADMQQVLEQQLVGCQAMLTEIEHKVAFLLETCKDQGLGDNGATQHEAEALSLKLKTVKCNLEKVQMMLQEKHSEDQ |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SYNE2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Nesprin 2 Antibody [NBP2-38620] - Immunofluorescent staining of human cell line MCF7 shows localization to nuclear membrane & intermediate filaments.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38620-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nesprin 2 Antibody [NBP2-38620] - Immunofluorescent staining of human cell line MCF7 shows localization to nuclear membrane & intermediate filaments.")
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38620-img0013.jpg "Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38620-img0009.jpg "Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human cerebral cortex shows no positivity in neurons as expected.")
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human kidney shows moderate to strong positivity in nuclear membrane in cells in tubules.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38620-img0011.jpg "Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human kidney shows moderate to strong positivity in nuclear membrane in cells in tubules.")
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human skin shows moderate positivity in nuclear membrane in squamous epithelial cells.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38620-img0012.jpg "Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-38620] - Staining of human skin shows moderate positivity in nuclear membrane in squamous epithelial cells.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")