Neprilysin/CD10 Antibody (CL12448) - Azide and BSA Free

Analysis in human small intestine and pancreas tissues using NBP3-44364 antibody. Corresponding Neprilysin/CD10 RNA-seq data are presented for the same tissues.

Staining of human kidney shows strong apical membranous positivity in cells in tubules and cells in glomeruli.

Staining of human pancreas shows very weak positivity in exocrine glandular cells.

Staining of human skin shows very weak positivity in squamous epithelial cells.

Staining of human small intestine shows strong apical membranous positivity in glandular cells.

• Reactivity: Hu
• Applications: IHC
• Clone: CL12448
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Neprilysin/CD10 Antibody (CL12448) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Neprilysin/CD10 Antibody (CL12448) - Azide and BSA Free (NBP3-15135) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P08473, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MME
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:2500-1:5000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Neprilysin/CD10 Antibody (CL12448) - Azide and BSA Free

• atriopeptidase
• CALLA
• CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase)
• CD10 antigen
• CD10
• CD10)
• CD10membrane metallo-endopeptidase variant 1
• Common acute lymphocytic leukemia antigen
• DKFZp686O16152
• EC 3.4.24
• EC 3.4.24.11
• Enkephalinase
• EPN
• Leu-19
• membrane metallo-endopeptidase variant 2
• membrane metallo-endopeptidase
• MGC126681
• MGC126707
• MME
• NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA
• Neprilysin
• Neutral Endopeptidase 24.11
• Neutral endopeptidase
• NKH1
• SFE
• Skin fibroblast elastase

Background

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MME