Neprilysin/CD10 Antibody (2A1H5E1)

Images

 
Western Blot: Neprilysin/CD10 Antibody (2A1H5E1) [NBP1-47356] - Western blot analysis using SOX9 Rabbit pAb against K562 (1), HCT116 (2), SW620 (3), LOVO (4), NTERA-2 (5), HEK293 (6) and HepG2 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: Neprilysin/CD10 Antibody (2A1H5E1) [NBP1-47356] - Immunofluorescence analysis of MCF-7 cells using SOX9 Rabbit pAb (red). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (2A1H5E1) [NBP1-47356] - Analysis of human breast ductal myoepithelium (A) and lymph tissue (B), showing cytoplasmic (A) and membrane (B) localization using CD10 ...read more
Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (2A1H5E1) [NBP1-47356] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SOX9 Rabbit pAb with DAB staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
2A1H5E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Neprilysin/CD10 Antibody (2A1H5E1) Summary

Immunogen
Purified recombinant fragment of human CD-10 expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MME
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1000
Application Notes
This antibody is useful in Immunohistochemistry-Paraffin, ELISA. Although not tested this antibody may work in Immunohistochemistry-Frozen.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Neprilysin/CD10 Antibody (2A1H5E1)

  • Atriopeptidase
  • CALLA
  • CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase)
  • CD10 antigen
  • CD10
  • CD10)
  • CD10membrane metallo-endopeptidase variant 1
  • Common acute lymphocytic leukemia antigen
  • DKFZp686O16152
  • EC 3.4.24
  • EC 3.4.24.11
  • Enkephalinase
  • EPN
  • Leu-19
  • membrane metallo-endopeptidase variant 2
  • membrane metallo-endopeptidase
  • MGC126681
  • MGC126707
  • MME
  • NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA
  • Neprilysin
  • Neutral Endopeptidase 24.11
  • Neutral endopeptidase
  • NKH1
  • SFE
  • Skin fibroblast elastase

Background

This antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALL). Subsequently, CD10 was shown to be the same molecule as the neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn2+-dependent metallo-peptidase with endothelin, glucagon, gastrin, neurotensin and bradykinin included among its substrates. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and expression has also been reported on mature B cells in germinal centres. In addition to the hematopoietic compartment, other major sites of CD10 expression are the brush border of enterocytes and renal tubules and glomeruli. There is partial blocking of CB-CALLA and MEM-78 monoclonal antibodies indicating that they recognize similar epitopes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MME
Entrez