NEK6 Antibody - BSA Free

Immunohistochemistry: NEK6 Antibody [NBP3-14415] - Immunohistochemistry of breast carcinoma

Immunohistochemistry-Paraffin: NEK6 Antibody [NBP3-14415] - Immunohistochemistry of formalin-fixed, paraffin-embedded human testis after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: NEK6 Antibody [NBP3-14415] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lymph node, non-Hodgkins lymphoma, after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: NEK6 Antibody [NBP3-14415] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lung, non-small cell carcinoma, after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: NEK6 Antibody [NBP3-14415] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, cortex, after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: NEK6 Antibody [NBP3-14415] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, glioblastoma, after heat-induced antigen retrieval.

Immunohistochemistry: NEK6 Antibody [NBP3-14415] - Immunohistochemistry of lung, non-small cell carcinoma,

• Reactivity: Hu, Pm, Pm-Or, Pm
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NEK6 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 14 amino acid peptide from N-Terminus of human NEK6.
• Epitope: N-Terminus
• Specificity: Human NEK6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Orangutan (100%), Monkey (100%), Primate (100%). Backed by our 100% Guarantee.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEK6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 7 ug/ml..

Reactivity Notes

Expected reactivity based on percent sequence identity: Mouse, Rat, Hamster, Canine, Equine, Porcine (86%)

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEK6 Antibody - BSA Free

• EC 2.7.11.1
• Never in mitosis A-related kinase 6
• NIMA (never in mitosis gene a)-related kinase 6
• NimA-related protein kinase 6
• Protein kinase SID6-1512
• putative serine-threonine protein kinase
• serine/threonine-protein kinase Nek6
• SID6-1512

Background

NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain. The protein is believed to regulate chromatin condensation in mammalian cells via phosphorylation of histones H1 and H3, and it has been shown to phosphorylate ribosomal S6 protein kinase (S6K1) and serum and glucocorticoid induced protein kinase (SGK1), which regulate diverse cellular processes. The protein also has been shown to bind NEK9, another NIMA-related kinase which regulates chromosome alignment and segregation. At least three mRNA transcripts have been identified, 1.6-, 2.6-, and 9.5-kb. The NEK6 gene is localized to 9q33-34, a region at which the loss of heterozygosity is associated with transitional cell carcinomas. NEK6 expression has been documented in many normal human tissues, including blood, brain, heart, liver, lung, skeletal muscle, spleen and testis. ESTs have been isolated from numerous tissue libraries, especially normal cervix and amnion and cancerous bone marrow.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK6