NEK4 Antibody

Western Blot: NEK4 Antibody [NBP1-42687] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NEK4 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NEK4 was also immunoprecipitated by rabbit anti-NEK4 antibody NBP1-42686, which recognizes an upstream epitope.

Immunohistochemistry: NEK4 Antibody [NBP1-42687] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NEK4 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

NEK4 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 475 and 525 of human NIMA (never in mitosis gene a)-related kinase 4 using the numbering given in entry NP_003148.2 (GeneID 6787).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEK4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEK4 Antibody

• EC 2.7.11
• Never in mitosis A-related kinase 4
• NIMA (never in mitosis gene a)-related kinase 4
• NimA-related protein kinase 4
• NRK2EC 2.7.11.1
• pp12301
• serine/threonine kinase 2
• serine/threonine protein kinase-2
• Serine/threonine-protein kinase 2
• serine/threonine-protein kinase Nek4
• Serine/threonine-protein kinase NRK2
• STK2MGC33171

Background

Belonging to the NEK Ser/Thr protein kinase family, NEK4 plays a significant role in the cell cycle, cell division, mitosis and protein phosphorylation process. More specifically, NEK4 can be found in the nucleus, and acts exclusively upon threonine residues. Molecular Functions of NEK4 include ATP binding, metal ion binding and protein serine/threonine kinase activity. Common diseases for NEK4 include adenocarcinoma, cancer, polycystic ovary syndrome, epithelial neoplasia, coffin-lowry syndrome, bipolar disorder, retinitis and malaria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK4
• Entrez:
  • 6787(Human)
• Uniprot:
  • P51957(Human)