NEK1 Antibody

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody diluted at 1:1000.

U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nek1 protein stained by Nek1 antibody diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 μm.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody diluted at 1:1000.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NEK1 antibody (NBP3-48625) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NEK1 Antibody Summary

• Description: Novus Biologicals Rabbit NEK1 Antibody (NBP3-48625) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NEK1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEK1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation Assay dependent
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 143 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for NEK1 Antibody

• DKFZp686D06121
• DKFZp686K12169
• EC 2.7.11
• EC 2.7.11.1
• KIAA1901MGC138800
• Never in mitosis A-related kinase 1
• NIMA (never in mitosis gene a)-related kinase 1
• NimA-related protein kinase 1
• NY-REN-55
• protein-serine/threonine kinase
• Renal carcinoma antigen NY-REN-55
• serine/threonine-protein kinase Nek1
• SRPS2

Background

Belonging to the NEK Ser/Thr protein kinase family, NEK1 plays an important role in the cell by responding to DNA damage. Molecular functions for NEK1 include protein serine/threonine kinase activity, protein tyrosine kinase activity, protein binding and ATP binding. More specifically, NEK1 is involved in cell cycle regulation, and the protein encoded by this gene is a serine/threonine kinase which is found in a centrosomal complex with FEZ1. Additionally, NEK1 also appears to possess tyrosine kinase activity and is involved in the control of meiosis and cilium assembly. NEK1 is known to interact with FEZ1, FEZ2, KIF3A, TSC2 and MRE11A. Common diseases for NEK1 include adenocarcinoma, cancer, short rib-polydactyly syndrome II and epithelial neoplasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK1