Nectin-4/PVRL4 Antibody (337516) [Phycoerythrin]

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Nectin-4 PE-conjugated Monoclonal Antibody (Catalog # FAB2659P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 337516
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Nectin-4/PVRL4 Antibody (337516) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Nectin-4
  Gly27-Val351
  Accession # Q96NY8
• Specificity: Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, does not cross-react with recombinant human Nectin‑1, -2, -3, or recombinant mouse Nectin-4.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PVRL4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nectin-4/PVRL4 Antibody (337516) [Phycoerythrin]

• EDSS1
• Ig superfamily receptor LNIR
• LNIR
• LNIRnectin 4
• Nectin4
• Nectin-4
• poliovirus receptor-related 4
• poliovirus receptor-related protein 4
• PRR4
• PRR4DKFZp686K05193
• PVRL4

Background

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nectin-4/PVRL4 Antibody (FAB2659P)(10)

Publications using FAB2659P

• Miguel Ángel Muñoz-Alía, Rebecca A. Nace, Lianwen Zhang, Stephen J. Russell Serotypic evolution of measles virus is constrained by multiple co-dominant B cell epitopes on its surface glycoproteins Cell Reports Medicine 4/1/2021 [PMID: 33948566]
• CD Zhang, YL Wang, DM Zhou, MY Zhu, Y Lv, XQ Hao, CF Qu, Y Chen, WZ Gu, BQ Wu, PC Chen, ZY Zhao A recombinant Chinese measles virus vaccine strain rMV-Hu191 inhibits human colorectal cancer growth through inducing autophagy and apoptosis regulating by PI3K/AKT pathway Translational Oncology, 2021-04-10;14(7):101091. 2021-04-10 [PMID: 33848808] (Flow Cytometry, Western Blot, Human)
• MÁ Muñoz-Alía, RA Nace, A Tischer, L Zhang, ES Bah, M Auton, SJ Russell Mev-stealth: A cd46-specific oncolytic measles virus resistant to neutralization by measles-immune human serum PloS Pathogens, 2021-02-03;17(2):e1009283. 2021-02-03 [PMID: 33534834] (Flow Cytometry, Human)
• BM Laksono, P Fortugno, BM Nijmeijer, RD de Vries, S Cordisco, T Kuiken, TBH Geijtenbee, WP Duprex, F Brancati, RL de Swart Measles skin rash: Infection of lymphoid and myeloid cells in the dermis precedes viral dissemination to the epidermis PLoS Pathog, 2020-10-08;16(10):e1008253. 2020-10-08 [PMID: 33031460] (Flow Cytometry, Human)
• T Hiramoto, M Tahara, J Liao, Y Soda, Y Miura, R Kurita, H Hamana, K Inoue, H Kohara, S Miyamoto, Y Hijikata, S Okano, Y Yamaguchi, Y Oda, K Ichiyanagi, H Toh, H Sasaki, H Kishi, A Ryo, A Muraguchi, M Takeda, K Tani Non-transmissible MV Vector with Segmented RNA Genome Establishes Different Types of iPSCs from Hematopoietic Cells Mol. Ther., 2019-09-12;28(1):129-141. 2019-09-12 [PMID: 31677955] (Flow Cytometry, Human)
• MA Muñoz-Alía, SJ Russell Probing Morbillivirus Antisera Neutralization Using Functional Chimerism between Measles Virus and Canine Distemper Virus Envelope Glycoproteins Viruses, 2019-07-27;11(8):. 2019-07-27 [PMID: 31357579] (Flow Cytometry, Human)
• G Gourru-Les, C Mathieu, T Thevenet, V Guillaume-, JF Jégou, CG Boer, K Tomczak, LM Bloyet, C Giraud, S Grande, C Goujon, C Cornu, B Horvat Measles virus infection of human keratinocytes: Possible link between measles and atopic dermatitis J. Dermatol. Sci, 2017-02-10;0(0):. 2017-02-10 [PMID: 28233587] (Flow Cytometry, Human)
• Manish R. Patel, Blake A. Jacobson, Holly Belgum, Ahmad Raza, Ahad Sadiq, Jeremy Drees et al. Measles Vaccine Strains for Virotherapy of Non–Small-Cell Lung Carcinoma Journal of Thoracic Oncology 2014-08-27 [PMID: 25157763] (Flow Cytometry, Human)
• Zhang Y, Patel B, Dey A, Ghorani E, Rai L, Elham M, Castleton AZ, Fielding AK Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function. J. Immunol., 2011-12-16;188(3):1002-10. 2011-12-16 [PMID: 22180616] (Flow Cytometry, Human)
• Noyce RS, Bondre DG, Ha MN Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus. PLoS Pathog., 2011-08-25;7(8):e1002240. 2011-08-25 [PMID: 21901103] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: PVRL4
• Uniprot:
  • Q96NY8()