Nectin-4/PVRL4 Antibody (337516) [Allophycocyanin]

MCF-7 human breast cancer cell line were stained with Mouse Anti-Human Nectin-4 APC-conjugated Monoclonal Antibody (Catalog # FAB2659A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 337516
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Nectin-4/PVRL4 Antibody (337516) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Nectin-4
  Gly27-Val351
  Accession # Q96NY8
• Specificity: Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, does not cross-react with recombinant human Nectin‑1, -2, -3, or recombinant mouse Nectin-4.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PVRL4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nectin-4/PVRL4 Antibody (337516) [Allophycocyanin]

• EDSS1
• Ig superfamily receptor LNIR
• LNIR
• LNIRnectin 4
• Nectin4
• Nectin-4
• poliovirus receptor-related 4
• poliovirus receptor-related protein 4
• PRR4
• PRR4DKFZp686K05193
• PVRL4

Background

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nectin-4/PVRL4 Antibody (FAB2659A)(1)

Publication using FAB2659A

• Kanako Moritoh, Koichiro Shoji, Yosuke Amagai, Tomoko Fujiyuki, Hiroki Sato, Misako Yoneda et al. Immune response elicited in the tumor microenvironment upon rMV‐SLAMblind cancer virotherapy Cancer Science 2023-05-01 [PMID: 36715555]

Bioinformatics

• Gene Symbol: PVRL4
• Uniprot:
  • Q96NY8()