Nectin-4/PVRL4 Antibody (337516) [Unconjugated]

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 337516
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Nectin-4/PVRL4 Antibody (337516) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Nectin-4
  Gly27-Val351
  Accession # Q96NY8
• Specificity: Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, does not cross-react with recombinant human Nectin‑1, -2, -3, or recombinant mouse Nectin-4.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PVRL4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nectin-4/PVRL4 Antibody (337516) [Unconjugated]

• EDSS1
• Ig superfamily receptor LNIR
• LNIR
• LNIRnectin 4
• Nectin4
• Nectin-4
• poliovirus receptor-related 4
• poliovirus receptor-related protein 4
• PRR4
• PRR4DKFZp686K05193
• PVRL4

Background

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nectin-4/PVRL4 Antibody (MAB2659)(7)

Publications using MAB2659

• Adi Reches, Yael Ophir, Natan Stein, Inbal Kol, Batya Isaacson, Yoav Charpak Amikam et al. Nectin4 is a novel TIGIT ligand which combines checkpoint inhibition and tumor specificity Journal for ImmunoTherapy of Cancer 6/4/2020 [PMID: 32503945]
• AR Generous, OJ Harrison, RB Troyanovsk, M Mateo, CK Navaratnar, RC Donohue, CK Pfaller, O Alekhina, AP Sergeeva, I Indra, T Thornburg, I Kochetkova, DD Billadeau, MP Taylor, SM Troyanovsk, B Honig, L Shapiro, R Cattaneo Trans-endocytosis elicited by nectins transfers cytoplasmic cargo including infectious material between cells J. Cell. Sci., 2019-08-23;0(0):. 8/23/2019 [PMID: 31331966]
• PC Buchanan, KL Boylan, B Walcheck, R Heinze, MA Geller, PA Argenta, AP Skubitz Ectodomain Shedding of the Cell Adhesion Molecule Nectin-4 in Ovarian Cancer is Mediated by ADAM10 and ADAM17 J. Biol. Chem, 2017-02-23;0(0):. 2017-02-23 [PMID: 28232483] (Flow Cytometry, Human)
• Kristin L.M. Boylan, Petra C. Buchanan, Rory D. Manion, Dip M. Shukla, Kelly Braumberger, Cody Bruggemeyer et al. The expression of Nectin-4 on the surface of ovarian cancer cells alters their ability to adhere, migrate, aggregate, and proliferate Oncotarget 2/7/2017 [PMID: 28038455]
• Galanis E, Atherton P, Maurer M, Knutson K, Dowdy S, Cliby W, Haluska P, Long H, Oberg A, Aderca I, Block M, Bakkum-Gamez J, Federspiel M, Russell S, Kalli K, Keeney G, Peng K, Hartmann L Oncolytic measles virus expressing the sodium iodide symporter to treat drug-resistant ovarian cancer. Cancer Res, 2014-11-14;75(1):22-30. 11/14/2014 [PMID: 25398436]
• Pavlova N, Pallasch C, Elia A, Braun C, Westbrook T, Hemann M, Elledge S A role for PVRL4-driven cell-cell interactions in tumorigenesis. Elife, 2013-04-30;2(0):e00358. 2013-04-30 [PMID: 23682311] (Functional Assay, Human)
• Derycke MS, Pambuccian SE, Gilks CB Nectin 4 overexpression in ovarian cancer tissues and serum: potential role as a serum biomarker. Am. J. Clin. Pathol., 2010-11-01;134(5):835-45. 2010-11-01 [PMID: 20959669] (Flow Cytometry, Western Blot, Human)

Bioinformatics

• Gene Symbol: PVRL4
• Uniprot:
  • Q96NY8()