Nectin-4/PVRL4 Antibody (337516) [Alexa Fluor® 488]

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Nectin-4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2659G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 337516
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Nectin-4/PVRL4 Antibody (337516) [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Nectin-4
  Gly27-Val351
  Accession # Q96NY8
• Specificity: Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, does not cross-react with recombinant human Nectin‑1, -2, -3, or recombinant mouse Nectin-4.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PVRL4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nectin-4/PVRL4 Antibody (337516) [Alexa Fluor® 488]

• EDSS1
• Ig superfamily receptor LNIR
• LNIR
• LNIRnectin 4
• Nectin4
• Nectin-4
• poliovirus receptor-related 4
• poliovirus receptor-related protein 4
• PRR4
• PRR4DKFZp686K05193
• PVRL4

Background

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nectin-4/PVRL4 Antibody (FAB2659G)(1)

Publication using FAB2659G

• A Floerching, JE Klein, MSC Finkbeiner, TE Schäfer, G Fuchs, J Doerner, H Zirngibl, M Ackermann, HM Kvasnicka, KA Chester, D Jäger, CR Ball, G Ungerechts, CE Engeland A vector-encoded bispecific killer engager to harness virus-activated NK cells as anti-tumor effectors Cell Death & Disease, 2023-02-10;14(2):104. 2023-02-10 [PMID: 36765035] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: PVRL4
• Uniprot:
  • Q96NY8()