Nectin-4/PVRL4 Antibody (337516) [Alexa Fluor® 700]

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Nectin-4 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB2659N, filled histogram) or isotype control antibody (Catalog # IC0041N, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 337516
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Nectin-4/PVRL4 Antibody (337516) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Nectin-4
  Gly27-Val351
  Accession # Q96NY8
• Specificity: Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, does not cross-react with recombinant human Nectin‑1, -2, -3, or recombinant mouse Nectin-4.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PVRL4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nectin-4/PVRL4 Antibody (337516) [Alexa Fluor® 700]

• EDSS1
• Ig superfamily receptor LNIR
• LNIR
• LNIRnectin 4
• Nectin4
• Nectin-4
• poliovirus receptor-related 4
• poliovirus receptor-related protein 4
• PRR4
• PRR4DKFZp686K05193
• PVRL4

Background

Nectin-4 is a 66-70 kDa member of the nectin family, Ig superfamily of molecules. Mature Nectin-4 is a 479 amino acid (aa) Type I transmembrane glycoprotein that contains one V-type and two C2-type Ig-like domains. Nectins mediate the Ca⁺⁺-independent cell adhesion in junctional complexes and connect to the Actin cytoskeleton via Afadin. Nectins are considered to first form loose cis-homodimers which aggregate and generate homooligomer in-trans, but preferentially generates Nectin-4:Nectin-1 heterooligomers. Cells known to express Nectin-4 include keratinocytes, transitional epithelium, CD49f⁺ CD24⁺ luminal mammary epithelium, tooth stratum intermedium cells, and respiratory pseudostratified ciliated columnar epithelium. It is also found on multiple carcinoma cell types. Nectin-4 can be cleaved to generate a 43-44 kDa soluble form that apparently appears only during tumorigenesis. Nectin-4 is also known to bind the measles virus, and possibly interact, indirectly or as a heterodimer, with the prolactin receptor. Over aa 27-351, human and mouse Nectin-4 share 90% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PVRL4
• Uniprot:
  • Q96NY8()