Ndufs1 Antibody - BSA Free

Western Blot: Ndufs1 Antibody [NBP3-12701] - Lane A: Raji Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:79 kDaObserved band size:72 kDa

Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP3-12701] - Staining of human NDUFS1 in human testis with rabbit polyclonal antibody at 1:1000 dilution.

Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP3-12701] - Staining of human NDUFS1 in human liver with rabbit polyclonal antibody at 1:1000 dilution.

Immunoprecipitation: Ndufs1 Antibody [NBP3-12701] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-NDUFS1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NDUFS1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 79 kDaObserved band size :72 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ndufs1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human Ndufs1 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFS1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Ndufs1 Antibody - BSA Free

• CI-75k
• CI-75kD
• complex I 75kDa subunit
• complex I, mitochondrial respiratory chain, 75-kD subunit
• Complex I-75kD
• EC 1.6.5.3
• EC 1.6.99.3
• EC 7.1.1.2
• MGC26839
• mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit
• NADH dehydrogenase (ubiquinone) Fe-S protein 1 (75kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Qreductase)
• NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
• Ndufs1
• PRO1304

Background

Ndufs1 is encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFS1