NDUFS6 Antibody

Western Blot: NDUFS6 Antibody [NBP1-49831] - (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: NDUFS6 Antibody [NBP1-49831] - 5ug/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: NDUFS6 Antibody [NBP1-49831] - Analysis of Mouse (A) and Rat (B) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NDUFS6 Antibody Summary

• Immunogen: Peptide with sequence C-RIRFVGRQKEVNEN corresponding to internal region according to NP_004544.1.
• Epitope: RIRFVGRQKEVNEN - Internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NDUFS6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.01-0.03ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1:100-1:2000
• Application Notes: Western blot: Approx 15kDa band observed in Human Heart lysates and 14kDa in Mouse and Rat Heart lysates (calculated MW of 13.7kDa according to NP_004544.1). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Kidney.

Reactivity Notes

Expected from sequence similarity: Canine, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NDUFS6 Antibody

• CI13KDA
• Complex I-13kD-A
• mitochondrial respiratory chain, 13-kD subunit
• NADH dehydrogenase (ubiquinone) Fe-S protein 6 (13kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Qreductase)
• NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
• NADH:ubiquinone oxidoreductase NDUFS6 subunit
• NADH-ubiquinone oxidoreductase 13 kDa-A subunit

Background

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction is made up of 7 subunits, including NDUFS6. See NDUFS1 (MIM 157655).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDUFS6 Antibody (NBP1-49831)(1)

Publication using NBP1-49831

• Saito A, Kawamoto M, Kamatani N. Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects. J Hum Genet 2009-06-01 [PMID: 19343046]

Bioinformatics

• Gene Symbol: NDUFS6
• Entrez:
  • 4726(Human)
  • 407785(Mouse)
  • 29478(Rat)
• Uniprot:
  • NP_004544.1(Human)