Ndufs4 Antibody - BSA Free

Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample(30ug whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Ndufs4 Antibody [NBP1-31465] - Analysis of methanol-fixed HeLa, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: Ndufs4 Antibody [NBP1-31465] - Human ovarian cancer, using NDUFS4 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.

Western Blot: Ndufs4 Antibody [NBP1-31465] - 50 ug Rat heart lysate/extract 12% SDS-PAGE gel, antibody dilution 1:1000.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ndufs4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Ndufs4 Antibody - BSA Free (NBP1-31465) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Ndufs4 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Ndufs4. The exact sequence is proprietary.
• Localization: Mitochondrion inner membrane, Peripheral membrane protein, Matrix side
• Predicted Species: Chimpanzee (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFS4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (80%), Bovine (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Ndufs4 Antibody - BSA Free

• AQDQ
• AQDQmitochondrial
• CI-18 kDa
• NADH dehydrogenase (ubiquinone) Fe-S protein 4 (18kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Qreductase)
• NADH-coenzyme Q reductase, 18-KD
• NADH-ubiquinone oxidoreductase 18 kDa subunit

Background

This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Ndufs4 Antibody (NBP1-31465)(3)

Publications using NBP1-31465

• Corra S, Cerutti R, Balmaceda V et al. Double administration of self-complementary AAV9NDUFS4 prevents Leigh disease in Ndufs4-/- mice Brain 2022-01-01 [PMID: 36270002] (IHC-P, WB, Mouse)
• Yang T, Wang Y, Liao W Et al. Down-regulation of EPB41L4A-AS1 mediated the brain aging and neurodegenerative diseases via damaging synthesis of NAD+ and ATP Research Square 2021-09-29 [PMID: 34758883] (WB)
• Silva-Pinheiro P, Cerutti R, Luna-Sanchez M et al. A Single Intravenous Injection of AAV-PHP.B-hNDUFS4 Ameliorates the Phenotype of Ndufs4 -/- Mice Mol Ther Methods Clin Dev 2020-06-12 [PMID: 32478122] (IHC-P, WB, Human)

Bioinformatics

• Gene Symbol: NDUFS4