NDUFA8 Antibody - Azide and BSA Free

Western Blot: NDUFA8 Antibody [NBP2-93802] - Analysis of 200ug extracts of 293T cells, using NDUFA8 at a dilition of 1:1000.

Immunocytochemistry/Immunofluorescence: NDUFA8 Antibody [NBP2-93802] - Immunofluorescence analysis of L929 cells using NDUFA8 Rabbit pAb (NBP2-93802) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western Blot: NDUFA8 Antibody [NBP2-93802] - Analysis of extracts of various cell lines, using NDUFA8 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

Immunocytochemistry/Immunofluorescence: NDUFA8 Antibody [NBP2-93802] - Immunofluorescence analysis of H9C2 cells using NDUFA8 Rabbit pAb (NBP2-93802) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NDUFA8 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit NDUFA8 Antibody - Azide and BSA Free (NBP2-93802) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-172 of human NDUFA8 (NP_055037.1). GAQCDKPNKEFMLCRWEEKDPRRCLEEGKLVNKCALDFFRQIKRHCAEPFTEYWTCIDYTGQQLFRHCRKQQAKFDECVLDKLGWVRPDLGELSKVTKVKTDRPLPENPYHSRPRPDPSPEIEGDLQPATHGSRFYFWTK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFA8
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunoprecipitation 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for NDUFA8 Antibody - Azide and BSA Free

• CI-19KD
• CI-PGIV
• complex I PGIV subunit
• Complex I-19kD
• Complex I-PGIV
• MGC793
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 (19kD, PGIV)
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa
• NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
• NADH:ubiquinone oxidoreductase PGIV subunit
• NADH-ubiquinone oxidoreductase 19 kDa subunit
• PGIV

Background

NDUFA8 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: NDUFA8