NDUFS5 Antibody - BSA Free

Western Blot: NDUFS5 Antibody [NBP3-12617] - Lane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:13 kDaObserved band size:13 kDa

Immunohistochemistry-Paraffin: NDUFS5 Antibody [NBP3-12617] - Staining of human NDUFS5 in human liver with rabbit polyclonal antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: NDUFS5 Antibody [NBP3-12617] - Staining of human NDUFS5 in human kidney with rabbit polyclonal antibody at 1:500 dilution.

Immunoprecipitation: NDUFS5 Antibody [NBP3-12617] - Lane A:0.5 mg K562 Whole Cell Lysate. 4 uL anti-NDUFS5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NDUFS5 rabbit polyclonal antibody,at 1:100 dilution .Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 13 kDaObserved band size :13 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NDUFS5 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human NDUFS5 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFS5
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1-5uL/mg of uLysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for NDUFS5 Antibody - BSA Free

• CI-15 kDa
• CI15K
• CI-15k
• Complex I-15 kDa
• NADH dehydrogenase (ubiquinone) Fe-S protein 5 (15kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Qreductase)
• NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
• NADH:ubiquinone oxidoreductase 15 kDa IP subunit
• NADH-ubiquinone oxidoreductase 15 kDa subunit

Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFS5