NDUFA5 Antibody

Western Blot: NDUFA5 Antibody [NBP3-03243] - Western blot analysis of extracts from normal (control) and NDUFA5 knockout (KO) 293T cells, using NDUFA5 antibody (NBP3-03243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry/Immunofluorescence: NDUFA5 Antibody [NBP3-03243] - Analysis of NIH-3T3 cells using NDUFA5 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: NDUFA5 Antibody [NBP3-03243] - Paraffin-embedded human gastric cancer using NDUFA5 antibody at dilution of 1:100 (40x lens).

Western Blot: NDUFA5 Antibody [NBP3-03243] - Analysis of extracts from normal (control) and NDUFA5 knockout (KO) HeLa cells, using NDUFA5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western Blot: NDUFA5 Antibody [NBP3-03243] - Western blot analysis of extracts of various cell lines, using NDUFA5 antibody (NBP3-03243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry-Paraffin: NDUFA5 Antibody [NBP3-03243] - Paraffin-embedded human tonsil using NDUFA5 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: NDUFA5 Antibody [NBP3-03243] - Paraffin-embedded human stomach using NDUFA5 antibody at dilution of 1:100 (40x lens).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NDUFA5 Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFA5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot
• Theoretical MW: 13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NDUFA5 Antibody

• B13,5 (13kD, B13)
• DKFZp781K1356
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
• UQOR13FLJ12147

Background

The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFA5