NDUFA2 Knockout HeLa Cell Lysate

Images

 
There are currently no images for NDUFA2 Lysate (NBP2-66200).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
LYOPH

Order Details

NDUFA2 Knockout HeLa Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,-2 bp deletion in exon1 and -1 bp deletion in exon1
Gene
NDUFA2

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout HeLa Cell

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for NDUFA2 Knockout HeLa Cell Lysate

  • B8
  • CD14
  • CIB8
  • CI-B8
  • complex I B8 subunit
  • Complex I-B8
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2 (8kD, B8)
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa
  • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
  • NADH-ubiquinone oxidoreductase B8 subunit
  • NADH-ubiquinone oxidoreductase subunit CI-B8

Background

The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, ELISA(Cap), Flow-CS, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
870-LP/CF
Species: Hu
NB100-56722
Species: Hu, Ca
Applications: WB, Flow, ICC/IF, IHC-P, IP, B/N, Flow-CS
NB110-89474
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Single Cell Western
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
MAB2546
Species: Hu
Applications: Flow, CyTOF-ready
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
NBP2-24867
Species: Hu, Mu
Applications: Flow, Func, In vitro, In vivo, CyTOF-ready, Flow-CS
NBP1-27946
Species: Mu
Applications: Flow, IHC-Fr, IP, B/N
NB100-77417
Species: Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
NB100-56655
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc

Publications for NDUFA2 Lysate (NBP2-66200) (0)

There are no publications for NDUFA2 Lysate (NBP2-66200).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NDUFA2 Lysate (NBP2-66200) (0)

There are no reviews for NDUFA2 Lysate (NBP2-66200). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NDUFA2 Lysate (NBP2-66200) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for NDUFA2 Lysate (NBP2-66200)

Discover related pathways, diseases and genes to NDUFA2 Lysate (NBP2-66200). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NDUFA2 Lysate (NBP2-66200)

Discover more about diseases related to NDUFA2 Lysate (NBP2-66200).
 

Pathways for NDUFA2 Lysate (NBP2-66200)

View related products by pathway.

PTMs for NDUFA2 Lysate (NBP2-66200)

Learn more about PTMs related to NDUFA2 Lysate (NBP2-66200).

Blogs on NDUFA2

There are no specific blogs for NDUFA2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

NDUFA2 Antibody
NBP1-88968

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NDUFA2 Knockout HeLa Cell Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol NDUFA2