NDUFA2 Knockout HeLa Cell Lysate Summary
Knockout achieved by using CRISPR/Cas9,-2 bp deletion in exon1 and -1 bp deletion in exon1
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.
Packaging, Storage & Formulations
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.
Lysate Details for Array
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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.
Alternate Names for NDUFA2 Knockout HeLa Cell Lysate
- complex I B8 subunit
- Complex I-B8
- NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2 (8kD, B8)
- NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa
- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
- NADH-ubiquinone oxidoreductase B8 subunit
- NADH-ubiquinone oxidoreductase subunit CI-B8
The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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