NDRG1 Antibody

Western blot shows lysates of human liver tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NDRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5209) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NDRG1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

NDRG1 Antibody Summary

• Immunogen: E. coli-derived recombinant human NDRG1
  Met111-Asp267
  Accession # Q92597
• Specificity: Detects human NDRG1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human N-myc is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NDRG1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NDRG1 Antibody

• CAP43
• CMT4D
• Differentiation-related gene 1 protein
• DRG1
• DRG-1
• DRG1HMSNL
• GC4
• HMSNL
• NDR1
• NDRG1
• Nickel-specific induction protein Cap43
• NMSL
• N-myc downstream regulated 1
• N-myc downstream-regulated gene 1 protein
• protein NDRG1
• protein regulated by oxygen-1
• PROXY1
• Reducing agents and tunicamycin-responsive protein
• RIT42
• RTP
• RTPGC4
• TARG1
• TDD5
• tunicamycin-responsive protein

Background

NDRG1 (N-myc downstream-regulated gene 1; also DRG-1 and Cap43) is a 43 kDa member of the NDRG family of proteins. It is ubiquitously expressed, found in fibroblasts, endothelial cells, respiratory epithelium and prostate epithelium. NDRG1 is highly expressed in nonproliferating, differentiating tissue. Human NDRG1 is 394 amino acids (aa) in length. It contains an NDGR domain (aa 286‑316) plus three tandem 10 aa hydrophilic repeats (aa 339‑368). There are three potential isoform variants. Two involve alternate start sites at Met82 and Met286, while a third shows a four aa substitution for aa 1‑34. Over aa 11‑267, human NDRG1 is 95% aa identical to mouse NDRG1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDRG1
• Uniprot:
  • Q92597()