NCK2 Antibody (S01-3C6)

Western Blot: NCK2 Antibody (S01-3C6) [NBP3-15063] - Western blot detection of NCK2 in K562, Rat Brain, C6, 3T3, Hela cell lysates using NCK2 Rabbit mAb (1:1000 diluted). Predicted band size: 43kDa. Observed band size: 43kDa.

Immunocytochemistry/Immunofluorescence: NCK2 Antibody (S01-3C6) [NBP3-15063] - Immunofluorescence of NCK2 (green) in Hela cells using NBP3-15063 at dilution 1/200, and DAPI (blue)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: S01-3C6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NCK2 Antibody (S01-3C6) Summary

• Description: Novus Biologicals Rabbit NCK2 Antibody (S01-3C6) (NBP3-15063) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human NCK2 (Uniprot#: O43639)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NCK2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/20-1/50
  • Western Blot 1/1000-1/5000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for NCK2 Antibody (S01-3C6)

• GRB4
• NCK adaptor protein 2cytoplasmic protein NCK2
• Nck-2
• NCKbeta
• noncatalytic region of tyrosine kinase, beta
• SH2/SH3 adaptor protein NCK-beta

Background

NCK2 encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCK2