NCK1 Antibody

Western Blot: Nck Antibody [NBP2-19489] - Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Nck Antibody [NBP2-19489] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Nck Antibody [NBP2-19489] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Pm, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NCK1 Antibody Summary

• Description: Novus Biologicals Rabbit NCK1 Antibody (NBP2-19489) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NCK1. The exact sequence is proprietary.
• Localization: Cytoplasm; Endoplasmic reticulum
• Predicted Species: Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCK1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NCK1 Antibody

• cytoplasmic protein NCK1
• MGC12668
• NCK adaptor protein 1melanoma NCK protein
• NCK1
• nck-1
• NCKalpha
• NCKNCK tyrosine kinase
• non-catalytic region of tyrosine kinase
• SH2/SH3 adaptor protein NCK-alpha

Background

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NCK1
• Uniprot:
  • P16333(Human)