| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33502-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33638-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22192-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98530-50ul | |||
![Western Blot: NCF1 Antibody [NB100-790] - Staining of U937 (A), Daudi (B), U251 (C) and negative control A431 (D) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.2, 0.01, and 0.3 ug/mL respectively. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NCF1-Antibody-Western-Blot-NB100-790-img0006.jpg "Western Blot: NCF1 Antibody [NB100-790] - Staining of U937 (A), Daudi (B), U251 (C) and negative control A431 (D) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.2, 0.01, and 0.3 ug/mL respectively. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: NCF1 Antibody [NB100-790] - Staining of paraffin embedded Human Colon. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NCF1-Antibody-Immunohistochemistry-Paraffin-NB100-790-img0004.jpg)
![Flow Cytometry: NCF1 Antibody [NB100-790] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/NCF1-Antibody-Flow-Cytometry-NB100-790-img0008.jpg)
![Western Blot: NCF1 Antibody [NB100-790] - Staining of Mouse Thymus (A) and Pig Spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NCF1-Antibody-Western-Blot-NB100-790-img0005.jpg)
![Immunofluorescence: NCF1 Antibody [NB100-790] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/NCF1-Antibody-Immunofluorescence-NB100-790-img0007.jpg)
![Western Blot: NCF1 Antibody [NBP2-26113] - Staining of Daudi cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NCF1-Antibody-Western-Blot-NBP2-26113-img0001.jpg "Western Blot: NCF1 Antibody [NBP2-26113] - Staining of Daudi cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: NCF1 Antibody [NBP2-26113] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/NCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-26113-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NCF1 Antibody [NBP2-26113] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/NCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-26113-img0002.jpg)
![Western Blot: NCF1 Antibody [NBP2-19486] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/NCF1-Antibody-Western-Blot-NBP2-19486-img0001.jpg "Western Blot: NCF1 Antibody [NBP2-19486] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: NCF1 Antibody [NBP2-19486] - Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NCF1 protein stained by NCF1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/NCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19486-img0005.jpg)
![Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-19486] - Hela xenograft, using NCF1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NCF1-Antibody-Immunohistochemistry-Paraffin-NBP2-19486-img0002.jpg)
![Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502] - Staining of human spleen shows high expression.](http://images.novusbio.com/fullsize/NCF1-Antibody-Immunohistochemistry-Paraffin-NBP2-33502-img0003.jpg "Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502] - Staining of human spleen shows high expression.")
![Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502] - Staining of human bone marrow shows moderate cytoplasmic positivity in a subset of hematopoietic cells.](http://images.novusbio.com/fullsize/NCF1-Antibody-Immunohistochemistry-Paraffin-NBP2-33502-img0001.jpg)
![Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/NCF1-Antibody-Immunohistochemistry-Paraffin-NBP2-33502-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502] - Staining in human spleen and cerebral cortex tissues using anti-NCF1 antibody. Corresponding NCF1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NCF1-Antibody-Immunohistochemistry-Paraffin-NBP2-33502-img0004.jpg)
![Western Blot: NCF1 Antibody [NBP2-19487] - Sample (50 ug of whole cell lysate) A: mouse spleen, 10% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/NCF1-Antibody-Western-Blot-NBP2-19487-img0002.jpg "Western Blot: NCF1 Antibody [NBP2-19487] - Sample (50 ug of whole cell lysate) A: mouse spleen, 10% SDS PAGE gel, diluted at 1:500.")
![Western Blot: NCF1 Antibody [NBP2-19487] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/NCF1-Antibody-Western-Blot-NBP2-19487-img0001.jpg)
![Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33638] - Staining of human small intestine shows strong cytoplasmic positivity in Paneth cells.](http://images.novusbio.com/fullsize/NCF1-Antibody-Immunohistochemistry-Paraffin-NBP2-33638-img0001.jpg "Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33638] - Staining of human small intestine shows strong cytoplasmic positivity in Paneth cells.")
![Western Blot: NCF1 Overexpression Lysate (Adult Normal) [NBL1-13509] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NCF1.](http://images.novusbio.com/fullsize/NCF1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13509-img0002.jpg "Western Blot: NCF1 Overexpression Lysate (Adult Normal) [NBL1-13509] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NCF1.")
![Western Blot: NCF1 Antibody (8J2F6) [NBP3-16572] - Western blot analysis of extracts of Raji cells, using NCF1 Rabbit mAb (NBP3-16572) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/NCF1-Antibody-8J2F6-Western-Blot-NBP3-16572-img0001.jpg "Western Blot: NCF1 Antibody (8J2F6) [NBP3-16572] - Western blot analysis of extracts of Raji cells, using NCF1 Rabbit mAb (NBP3-16572) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: NCF1 Antibody (SR1776) [NBP3-22192] - Western blot analysis using NBP3-22192 in Raji cell lysate.](http://images.novusbio.com/fullsize/nbp3-22192_rabbit-ncf1-mab-sr1776-6720239533773.jpg "Western Blot: NCF1 Antibody (SR1776) [NBP3-22192] - Western blot analysis using NBP3-22192 in Raji cell lysate.")
![Western Blot: NCF1 Antibody (A6B10-R) [NBP3-32696] - Western blot analysis of NCF1 on different lysates with Mouse anti-NCF1 antibody (NBP3-32696) at 1/2,000 dilution. Lane 1: Ramos cell lysate Lane 2: Raji cell lysate Lane 3: Daudi cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32696) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32696_mouse-ncf1-mab-a6b10-r-278202415295588.jpg "Western Blot: NCF1 Antibody (A6B10-R) [NBP3-32696] - Western blot analysis of NCF1 on different lysates with Mouse anti-NCF1 antibody (NBP3-32696) at 1/2,000 dilution. Lane 1: Ramos cell lysate Lane 2: Raji cell lysate Lane 3: Daudi cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32696) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![SDS-Page: Recombinant Human NCF1 His Protein [NBP1-45305] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-NCF1-His-Protein-SDS-Page-NBP1-45305-img0001.jpg "SDS-Page: Recombinant Human NCF1 His Protein [NBP1-45305] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-98530] - Immunochemical staining of human NCF1 in human spleen with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NCF1-Antibody-Immunohistochemistry-Paraffin-NBP2-98530-img0001.jpg "Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-98530] - Immunochemical staining of human NCF1 in human spleen with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.")