NCF1 Overexpression Lysate

Images

 
Western Blot: NCF1 Overexpression Lysate (Adult Normal) [NBL1-13509] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NCF1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NCF1 Overexpression Lysate Summary

Description
Transient overexpression lysate of neutrophil cytosolic factor 1 (NCF1) The lysate was created in HEK293T cells, using Plasmid ID RC201233 and based on accession number NM_000265. The protein contains a C-MYC/DDK Tag.
Gene
NCF1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
44.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NCF1 Overexpression Lysate

  • 47 kDa neutrophil oxidase factor
  • FLJ79451
  • NADPH oxidase organizer 2
  • NCF-1
  • NCF1A
  • neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein
  • neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1)
  • neutrophil cytosolic factor 1
  • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)
  • Neutrophil NADPH oxidase factor 1
  • Nox organizer 2
  • NOXO2NCF-47K
  • Nox-organizing protein 2
  • p47phox
  • SH3 and PX domain-containing protein 1A
  • SH3PXD1Ap47-phox

Background

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for NCF1 Lysate (NBL1-13509) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-13509

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Blogs on NCF1.

Identifying tumoral and stromal transcriptomes that underlie tumor plasticity and stromal neuroinflammatory response in brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Cancers in the brain often come from tumors elsewhere in the body. Several adaptive mechanisms influence brain metastasis, such as blood brain barrier leakage that can be induced by...  Read full blog post.

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Bioinformatics

Gene Symbol NCF1
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