NCAPD3 Antibody - BSA Free

Immunoprecipitation: NCAPD3 Antibody - BSA Free [NBP3-47379] - IP Result of anti-NCAPD3 (IP:, 4ug; Detection: 1:500) with HeLa cells lysate 600ug.

Western Blot: NCAPD3 Antibody - BSA Free [NBP3-47379] - HepG2 cells were subjected to SDS PAGE followed by western blot with (NCAPD3 antibody) at dilution of 1:800

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NCAPD3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NCAPD3 Antibody - BSA Free (NBP3-47379) is a polyclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: non-SMC condensin II complex, subunit D3. (Uniprot# P42695)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCAPD3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 1:200-1:2000
  • Western Blot 1:500-1:2000
• Theoretical MW: 165 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NCAPD3 Antibody - BSA Free

• CAPD3
• CAP-D3
• FLJ42888
• hCAP-D3condensin-2 complex subunit D3
• hcp-6
• hHCP-6
• KIAA0056MGC104671
• Non-SMC condensin II complex subunit D3
• non-SMC condensin II complex, subunit D3

Background

Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCAPD3