NCAM-1/CD56 Antibody (RNL-1) - BSA Free

Immunohistochemistry: NCAM-1/CD56 Antibody (RNL-1) [NBP1-97717] - H82 cells, derived from small cell lung cancer, showing strong positive staining for NCAM/CD56 at the sites of intracellular connections.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC
• Clone: RNL-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NCAM-1/CD56 Antibody (RNL-1) - BSA Free Summary

• Description: The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
• Immunogen: Derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with the small cell lung cancer cell line NCI-H82.
• Specificity: This antibody was defined as a cluster I antibody during the Second International Workshop on Small Cell Lung Cancer (SCLC) Antibodies and recognizes the extracellular region of NCAM-1/CD56.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCAM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
• Application Notes: This antibody is suitable for immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with fluorochrome conjugated secondary antibodies or avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent..

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for NCAM-1/CD56 Antibody (RNL-1) - BSA Free

• CD56 / NCAM-1
• CD56 antigen
• CD56
• MSK39
• NCAM1
• N-CAM-1
• NCAM-1
• NCAMantigen recognized by monoclonal 5.1H11
• neural cell adhesion molecule 1
• neural cell adhesion molecule, NCAM

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for NCAM-1/CD56 Antibody (NBP1-97717). (Showing 1 - 1 of 1 FAQ).

1. I see you advertise NCAM Antibody (RNL-1) (NBP1-97717) as being used for recognizing mouse NCAM, but can one use a mouse monoclonal on mouse tissue? How does that work?
   • Mouse on mouse staining, while not ideal, can still be accomplished. This antibody was raised against the human form of the protein, but most likely shares significant homology with mouse, to where it can also pick up that protein. To eliminate the high background that can occur with same species staining we would recommend directly conjugating the primary antibody to your detection method such as HRP, or a Fluorescent marker, to eliminate the use of a secondary. Without having to use a secondary you won't get non-specific binding of the anti-mouse secondary to your samples. Another way you can achieve this is by blocking endogenous Fc receptors with non-specific antibodies that are mouse. This can be accomplished by incubating your samples with mouse serum ahead of adding your antibodies. This can help block out the Fc receptors and eliminate the background if you plan to use a secondary.

Bioinformatics

• Gene Symbol: NCAM1