NAA10 Antibody

Western Blot: NAA10 Antibody [NBP2-19461] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and blotted with ARD1A antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: NAA10 Antibody [NBP2-19461] - Detection of NAA10 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: NAA10 protein stained by ARD1A antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry-Paraffin: NAA10 Antibody [NBP2-19461] - Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.

Western Blot: NAA10 Antibody [NBP2-19461] - Sample (30 ug of whole cell lysate) A: H1299 transfected scramble siRNA B: H1299 transfected ARDIA siRNA 12% SDS PAGE gel, diluted at 1:5000.

Immunoprecipitation: NAA10 Antibody [NBP2-19461] - Immunoprecipitation of ARD1A protein from HeLa whole cell extracts using 5 ug of ARD1A antibody. Western blot analysis was performed using ARD1A antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Bv, Rb
• Applications: WB, ICC/IF, IHC, IHC-P, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NAA10 Antibody Summary

• Immunogen: Full length human ARD1A Recombinant protein.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Rat (91%), Bovine (97%), Rabbit (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAA10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:10000
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for NAA10 Antibody

• ARD1 homolog A, N-acetyltransferase (S. cerevisiae)
• ARD1 homolog, N-acetyltransferase (S. cerevisiae)
• ARD1
• ARD1AARD1 homolog, N-acetyltransferase
• EC 2.3.1.-
• EC 2.3.1.88
• MGC71248
• N(alpha)-acetyltransferase 10, NatA catalytic subunit
• N-acetyltransferase ARD1, human homolog of
• N-acetyltransferase
• N-alpha-acetyltransferase 10, NatA catalytic subunit
• N-terminal acetyltransferase complex ARD1 subunit homolog A

Background

N-alpha-acetylation is one of the most common protein modifications that occurs during protein synthesis and involves the transfer of an acetyl group from acetyl-coenzyme A to the protein alpha-amino group. ARD1A, together with NATH (NARG1; MIM 608000), is part of a major N-alpha-acetyltransferase complex responsible for alpha-acetylation of proteins and peptides (Sanchez-Puig and Fersht, 2006 [PubMed 16823041]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NAA10 Antibody (NBP2-19461)(1)

Publication using NBP2-19461

• Simsek D, Tiu GC, Flynn RA et al. The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Cell. 2017-06-01 [PMID: 28575669]

Bioinformatics

• Gene Symbol: NAA10