NAA10 Antibody (3G3B9) - BSA Free

Western Blot: NAA10 Antibody (3G3B9) [NBP2-61891] - Analysis using NAA10 mAb against HEK293 (1) and NAA10 (AA: 111-235)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: NAA10 Antibody (3G3B9) [NBP2-61891] - Analysis of Hela cells using NAA10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Flow Cytometry: NAA10 Antibody (3G3B9) [NBP2-61891] - Analysis of SMMC-7721 cells using NAA10 mouse mAb (green) and negative control (red).

Western Blot: NAA10 Antibody (3G3B9) [NBP2-61891] - Analysis using NAA10 mAb against human NAA10 (AA: 111-235) recombinant protein. (Expected MW is 47.2 kDa)

Western Blot: NAA10 Antibody (3G3B9) [NBP2-61891] - Analysis using NAA10 mouse mAb against COS7 (1), HEK293 (2), HL-60 (3), MCF-7 (4), Hela (5), NIH/3T3 (6), and C2C12 (7) cell lysate.

ELISA: NAA10 Antibody (3G3B9) [NBP2-61891] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu, Mu, Pm
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 3G3B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NAA10 Antibody (3G3B9) - BSA Free Summary

• Description: Novus Biologicals Mouse NAA10 Antibody (3G3B9) - BSA Free (NBP2-61891) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human NAA10 (AA: 111-235) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAA10
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 26.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for NAA10 Antibody (3G3B9) - BSA Free

• ARD1 homolog A, N-acetyltransferase (S. cerevisiae)
• ARD1 homolog, N-acetyltransferase (S. cerevisiae)
• ARD1
• ARD1AARD1 homolog, N-acetyltransferase
• EC 2.3.1.-
• EC 2.3.1.88
• MGC71248
• N(alpha)-acetyltransferase 10, NatA catalytic subunit
• N-acetyltransferase ARD1, human homolog of
• N-acetyltransferase
• N-alpha-acetyltransferase 10, NatA catalytic subunit
• N-terminal acetyltransferase complex ARD1 subunit homolog A

Background

N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAA10