N-WASP [p Ser484, p Ser485] Antibody

Western Blot: N-WASP [p Ser484, p Ser485] Antibody [NBP2-29669] - Western blot of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 and 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 and 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 and 6).

• Reactivity: Hu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

N-WASP [p Ser484, p Ser485] Antibody Summary

• Description: Relevant Entrez Gene Numbers: NM_003941.2
• Immunogen: Phospho-N-WASP (S484/S485) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484.
• Epitope: Phosphoserine 484 / Phosphserine 485
• Modification: p Ser484, p Ser485
• Specificity: The antibody detects a 63 kDa protein corresponding to the molecular weight of phosphorylated WASP on SDS-PAGE immunoBlots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa protein corresponding to phosphorylated N-WASP.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WASL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA.
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Relevant Entrez Gene Numbers: NM_003941.2

Alternate Names for N-WASP [p Ser484, p Ser485] Antibody

• NWASP
• N-WASP
• N-WASPneural WiskNWASP
• TRS4
• WASL
• WiskDKFZp779G0847
• WiskMGC48327

Background

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Phosphorylation regulates the activity of both proteins. Dual phosphorylation of WASP on serine 483 and 484 by casein kinases increase the affinity for the ARP2/3 complex. Thus, dual serine phosphorylation may be important for formation of actin-based structures in various cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WASL
• Uniprot:
  • O00401()