N-WASP [p Tyr256] Antibody

Western Blot: N-WASP [p Tyr256] Antibody [NB600-1169] - Western blot analysis of control and pervanadate-treated A431 cells (20 ug/lane). Blots were probed with either rabbit polyclonal anti-N-WASP (Ser484/Ser485) or anti-N-WASP (Tyr256).

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

N-WASP [p Tyr256] Antibody Summary

• Immunogen: Phospho N WASP (Tyr 256) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around tyrosine 256 of human N WASP. The human WASP sequence has a similar peptide sequence surrounding tyrosine 291.
• Epitope: phosphotyrosine 256
• Modification: p Tyr256
• Localization: Cytoplasmic
• Specificity: N WASP [Tyr256]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WASL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000
  • Western Blot 1:10000
• Application Notes: In Western Blot a 65kDa band is seen. Optimal dilutions should be determined by the end user.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1 ml PBS, 1.0 BSA mg/ml and 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for N-WASP [p Tyr256] Antibody

• NWASP
• N-WASP
• N-WASPneural WiskNWASP
• TRS4
• WASL
• WiskDKFZp779G0847
• WiskMGC48327

Background

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with higher homology in the functional regions of these proteins. Phosphorylation at serine and tyrosine residues regulates the activity of both proteins. WASP is tyrosine phosphorylated at tyrosine 291 after antigen receptor activation in B-cells and collagen stimulation of platelets. Phosphorylation of the analogous site in N-WASP (Tyr256) stimulates its activity, reduces nuclear N-WASP, and is required for neurite extension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for N-WASP Antibody (NB600-1169)(1)

Publication using NB600-1169

• Bravo-Santano N, Stolting H, Cooper F et al. Host-directed kinase inhibitors act as novel therapies against intracellular Staphylococcus aureus Sci Rep 2019-03-19 [PMID: 30890742] (Block/Neutralize)

Bioinformatics

• Gene Symbol: WASL
• Entrez:
  • 8976(Human)