N myc interactor Antibody (9E8) - Azide and BSA Free

Western Blot: N myc interactor Antibody (9E8) [H00009111-M04] - Analysis of NMI expression in HeLa (Cat # L013V1).

Immunohistochemistry-Paraffin: N myc interactor Antibody (9E8) [H00009111-M04] - Analysis of monoclonal antibody to NMI on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml

ELISA: N myc interactor Antibody (9E8) [H00009111-M04] - Detection limit for recombinant GST tagged NMI is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 9E8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

N myc interactor Antibody (9E8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse N myc interactor Antibody (9E8) - Azide and BSA Free (H00009111-M04) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-N myc interactor Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: NMI (AAH01268, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEADKDDTQQILKEHSPDEFIKDEQNKGLIDEITKKNIQLKKEIQKLETELQEATKEFQIKEDIPETKMKFLSVETPENDSQLSNISCSFQVSSKVPYEI
• Specificity: NMI (9E8)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NMI
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:505
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA and immunohistochemistry (paraffin).

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for N myc interactor Antibody (9E8) - Azide and BSA Free

• Nmi
• N-myc (and STAT) interactor
• N-myc and STAT interactor
• N-myc interactor
• N-myc-interactor

Background

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for N myc interactor Antibody (H00009111-M04)(1)

Publication using H00009111-M04

• Saito Y, Yukawa A, Matozaki M et al. Nmi interacts with Hsp105b and enhances the Hsp105b-mediated Hsp70 expression. Exp Cell Res. 2014-07-23 [PMID: 25088258]

Bioinformatics

• Gene Symbol: NMI
• Entrez:
  • 9111(Human)
• OMIM:
  • 603525(Human)
• Uniprot:
  • AAH01268(Human)