Myosin 1C Antibody

Immunohistochemistry-Paraffin: Myosin 1C Antibody [NBP1-87745] - Staining in human lung and skeletal muscle tissues using anti-MYO1C antibody. Corresponding MYO1C RNA-seq data are presented for the same tissues.

Western Blot: Myosin 1C Antibody [NBP1-87745] - Analysis in human cell line A-549.

Immunohistochemistry-Paraffin: Myosin 1C Antibody [NBP1-87745] - Staining of human lung shows high expression.

Immunohistochemistry-Paraffin: Myosin 1C Antibody [NBP1-87745] - Staining of human skeletal muscle shows low expression as expected.

• Reactivity: Hu, Rt, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Myosin 1C Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GEEETLRRLGLERNPQSYLYLVKGQCAKVSSINDKSDWKVVRKALTVIDFTEDEVEDLLSIVASVLHLGNIHFAANEESNAQVTTENQLKYLTRLLSVEGSTLREALTHRKIIAKGEELLSPLNLEQAAYARDALAKAVYSRTFTWLV
• Predicted Species: Mouse (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYO1C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: WB reported in scientific literature (PMID: 26170120). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Myosin 1C Protein (NBP1-87745PEP)

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 26170120).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Myosin 1C Antibody

• FLJ23903
• MMIb
• MMI-beta
• Myosin I beta
• myosin IC
• myosin-I beta
• myosin-Ic
• myr2
• NMI
• nuclear myosin I

Background

Myosin 1C encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. The related gene myosin IE has been referred to as myosin IC in the literature, but it is a distinct locus on chromosome 19. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin 1C Antibody (NBP1-87745)(2)

Publications using NBP1-87745

• Hedberg Oldfors C, Dios DG, Linder A et al. Analysis of an independent tumor suppressor locus telomeric to Tp53 suggested Inpp5k and Myo1c as novel tumor suppressor gene candidates in this region. BMC Genet 2015-07-14 [PMID: 26170120] (WB, Rat)
• Tiwari A, Jung JJ, Inamdar SM et al. The myosin motor Myo1c is required for VEGFR2 delivery to the cell surface and for angiogenic signaling. Am J Physiol Heart Circ Physiol 2013-03-01 [PMID: 23262137]

Bioinformatics

• Gene Symbol: MYO1C