N myc interactor Antibody (9D8) - Azide and BSA Free

Western Blot: N myc interactor Antibody (9D8) [H00009111-M01] - NMI monoclonal antibody (M01), clone 9D8 Analysis of NMI expression in HeLa.

Immunohistochemistry-Paraffin: N myc interactor Antibody (9D8) [H00009111-M01] - Analysis of monoclonal antibody to NMI on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Western Blot: N myc interactor Antibody (9D8) [H00009111-M01] - Analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi ( Cat # H00009111-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NMI monoclonal antibody (M01), clone 9D8 (Cat # H00009111-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: N myc interactor Antibody (9D8) [H00009111-M01] - Analysis of NMI expression in transfected 293T cell line by NMI monoclonal antibody (M01), clone 9D8.Lane 1: NMI transfected lysate(35.1 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: N myc interactor Antibody (9D8) [H00009111-M01] - Detection limit for recombinant GST tagged NMI is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, KD
• Clone: 9D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

N myc interactor Antibody (9D8) - Azide and BSA Free Summary

• Immunogen: NMI (AAH01268, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEADKDDTQQILKEHSPDEFIKDEQNKGLIDEITKKNIQLKKEIQKLETELQEATKEFQIKEDIPETKMKFLSVETPENDSQLSNISCSFQVSSKVPYEI
• Specificity: NMI - N-myc (and STAT) interactor
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NMI
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for N myc interactor Antibody (9D8) - Azide and BSA Free

• Nmi
• N-myc (and STAT) interactor
• N-myc and STAT interactor
• N-myc interactor
• N-myc-interactor

Background

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NMI
• Entrez:
  • 9111(Human)
• OMIM:
  • 603525(Human)
• Uniprot:
  • AAH01268(Human)