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N myc interactor Antibody (9D8)

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Western Blot: N myc interactor Antibody (9D8) [H00009111-M01] - NMI monoclonal antibody (M01), clone 9D8 Analysis of NMI expression in HeLa.
Western Blot: N myc interactor Antibody (9D8) [H00009111-M01] - NMI monoclonal antibody (M01), clone 9D8 Analysis of NMI expression in HeLa.
Immunohistochemistry-Paraffin: N myc interactor Antibody (9D8) [H00009111-M01] - Analysis of monoclonal antibody to NMI on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: N myc interactor Antibody (9D8) [H00009111-M01] - Analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi ( Cat # H00009111-R01V ) (Lane 2) or non-transfected control ...read more
Western Blot: N myc interactor Antibody (9D8) [H00009111-M01] - Analysis of NMI expression in transfected 293T cell line by NMI monoclonal antibody (M01), clone 9D8.Lane 1: NMI transfected lysate(35.1 KDa).Lane 2: ...read more
Sandwich ELISA: N myc interactor Antibody (9D8) [H00009111-M01] - Detection limit for recombinant GST tagged NMI is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
9D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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N myc interactor Antibody (9D8) Summary

Immunogen
NMI (AAH01268, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag.MEADKDDTQQILKEHSPDEFIKDEQNKGLIDEITKKNIQLKKEIQKLETELQEATKEFQIKEDIPETKMKFLSVETPENDSQLSNISCSFQVSSKVPYEI
Specificity
NMI - N-myc (and STAT) interactor
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NMI
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for N myc interactor Antibody (9D8)

  • Nmi
  • N-myc (and STAT) interactor
  • N-myc and STAT interactor
  • N-myc interactor
  • N-myc-interactor

Background

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: Myosin 1C Antibody [NBP1-87745] - Staining in human lung and skeletal muscle tissues using anti-MYO1C antibody. Corresponding MYO1C RNA-seq data are presented for the same tissues.
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Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).
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Western Blot: IFI35 Antibody (1C9) [NBP2-01108] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFI35 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFI35.
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Immunohistochemistry-Paraffin: IFI35 Antibody (1C9) [NBP2-01108] - Staining of paraffin-embedded Human pancreas tissue using anti-IFI35 mouse monoclonal antibody.
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Western Blot: Frataxin Antibody (1C12) [NBP2-01743] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Frataxin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Frataxin.
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N myc interactor
Antibody (9D8)
H00009111-M01
N myc interactor Antibody (9D8)
Species: Hu
Applications: WB, ELISA, IHC, IHC-P

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Bioinformatics

Gene Symbol NMI
Entrez
OMIM
Uniprot