N-Acetylglucosaminyltransferase III/MGAT3 Antibody - BSA Free

Western Blot: N-Acetylglucosaminyltransferase III/MGAT3 Antibody - BSA Free [NBP2-93814] - Western blot analysis of Mouse kidney, using N-Acetylglucosaminyltransferase III/MGAT3 antibody (A8134) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

N-Acetylglucosaminyltransferase III/MGAT3 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 364-533 of human MGAT3 (NP_002400.3). GCTVDMLQAVYGLDGIRLRRRQYYTMPNFRQYENRTGHILVQWSLGSPLHFAGWHCSWCFTPEGIYFKLVSAQNGDFPRWGDYEDKRDLNYIRGLIRTGGWFDGTQQEYPPADPSEHMYAPKYLLKNYDRFHYLLDNPYQEPRSTAAGGWRHRGPEGRPPARGKLDEAEV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MGAT3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for N-Acetylglucosaminyltransferase III/MGAT3 Antibody - BSA Free

• beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• EC 2.4.1.144
• FLJ43371
• GGNT3
• GlcNAc-T III
• GNT3
• GNT-III
• GNT-IIIMGC142278
• mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• MGAT3
• MGC141943
• N-acetylglucosaminyltransferase III
• N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III

Background

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MGAT3