N-Acetylglucosaminyltransferase V/MGAT5 Antibody (706824)

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human N‑Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) ...read more
N‑Acetylglucosaminyltransferase V/MGAT5 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human N‑Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
706824
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

N-Acetylglucosaminyltransferase V/MGAT5 Antibody (706824) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human N-Acetylglucosaminyltransferase V/MGAT5
Leu189-Leu741
Accession # Q09328
Specificity
Detects human N-Acetylglucosaminyltransferase V/MGAT5 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MGAT5
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB5469 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for N-Acetylglucosaminyltransferase V/MGAT5 Antibody (706824)

  • alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
  • Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
  • EC 2.4.1.155
  • GGNT5
  • GlcNAc-T V
  • GNT-VGNT-VA
  • Mannoside acetylglucosaminyltransferase 5
  • mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
  • MGAT5
  • N-Acetylglucosaminyltransferase V
  • N-acetylglucosaminyl-transferase V

Background

N-Acetylglucosaminyltransferase V (GnT-V), also known as mannosylglycoprotein N-acetyl-glucosaminyltransferase 5 (MGAT5), adds an N-acetylglucosamine to the alpha 1-6-linked core mannose of an N-linked oligosaccharide in the Golgi apparatus (1). This reaction is the committing step for the biosynthesis of beta 1-6GlcNAc-branched arm in N-glycans. The degree of N-glycan branching has been shown to regulate cell proliferation and differentiation (2). An increase in the GnT-V activity and its glycan products is also known to positively correlate with the progression of invasive malignancies (3, 4). For example, ectopic expression of GnT-V in epithelial cells results in morphological transformation and tumor growth in mice and overexpression in carcinoma cells has been shown to induce metastatic spread (3-5).

  1. Saito, H. et al. (1994) Biochem. Biophys. Res. Commun. 198:318.
  2. Lau, K.S. et al. (2007) Cell 198:123.
  3. Dennis, J.W. et al. (2002) Biochim. Biophys. Acta 1573:414.
  4. Granovsky, M. et al. (2008) Nat. Med. 7:1.
  5. Kim, Y.S. et al. (2008) Mol. Cell. Proteomics 7:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for N-Acetylglucosaminyltransferase V/MGAT5 Antibody (MAB5469)(2)

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Bioinformatics

Gene Symbol MGAT5
Uniprot