N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

Images

 
Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-00190] - (0.01ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody Summary

Immunogen
Peptide with sequence C-TTHNLEDHTKLP corresponding to internal region according to NP_000503.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GALNS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human bone marrow and placenta lysates (calculated MW of 58.0 kDa band according to NP_000503.1).
Control
N-Acetylgalactosamine-6-Sulfatase/GALNS Overexpression Lysate
Publications
Read Publication using NBP1-00190.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

  • Chondroitinase
  • Chondroitinsulfatase
  • EC 3.1.6
  • EC 3.1.6.4
  • FLJ17434
  • FLJ42844
  • FLJ98217
  • GA6S
  • galactosamine (N-acetyl)-6-sulfate sulfatase
  • Galactose-6-Sulfate Sulfatase
  • GalNAc6S Sulfatase
  • GALNAC6S
  • GALNS
  • GAS
  • MPS4A
  • NAcetylgalactosamine6Sulfatase
  • N-Acetylgalactosamine-6-Sulfatase
  • N-acetylgalactosamine-6-sulfate sulfatase

Background

This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody (NBP1-00190)(1)

Reviews for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody (NBP1-00190) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody (NBP1-00190). (Showing 1 - 1 of 1 FAQ).

  1. I would like to know if this item, item number NBP1-00190, is very specific to N-linked glycosyl residues only
    • This antibody recognizes the peptide sequence TTHNLEDHTKLP (AA428-439) and is polyclonal, so it can recognize multiple places on this peptide. There is not a glycosylation site within those residues, so no, it is not specific to N-linked glycosyl residues.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody (NBP1-00190)

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Bioinformatics

Gene Symbol GALNS
Entrez
Uniprot