N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-00190] - (0.01ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody Summary

• Immunogen: Peptide with sequence C-TTHNLEDHTKLP corresponding to internal region according to NP_000503.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GALNS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human bone marrow and placenta lysates (calculated MW of 58.0 kDa band according to NP_000503.1).
• Control:

  N-Acetylgalactosamine-6-Sulfatase/GALNS Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

• Chondroitinase
• Chondroitinsulfatase
• EC 3.1.6
• EC 3.1.6.4
• FLJ17434
• FLJ42844
• FLJ98217
• GA6S
• galactosamine (N-acetyl)-6-sulfate sulfatase
• Galactose-6-Sulfate Sulfatase
• GalNAc6S Sulfatase
• GALNAC6S
• GALNS
• GAS
• MPS4A
• NAcetylgalactosamine6Sulfatase
• N-Acetylgalactosamine-6-Sulfatase
• N-acetylgalactosamine-6-sulfate sulfatase

Background

This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody (NBP1-00190)(1)

Publication using NBP1-00190

• Houle JD, Tom VJ, Mayes D et al. Combining an autologous peripheral nervous system bridge and matrix modification by chondroitinase allows robust, functional regeneration beyond a hemisection lesion of the adult rat spinal cord. J Neurosci 2006-07-12 [PMID: 16837588]

FAQs for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody (NBP1-00190). (Showing 1 - 1 of 1 FAQ).

1. I would like to know if this item, item number NBP1-00190, is very specific to N-linked glycosyl residues only
   • This antibody recognizes the peptide sequence TTHNLEDHTKLP (AA428-439) and is polyclonal, so it can recognize multiple places on this peptide. There is not a glycosylation site within those residues, so no, it is not specific to N-linked glycosyl residues.

Bioinformatics

• Gene Symbol: GALNS
• Entrez:
  • 2588(Human)
• Uniprot:
  • NP_000503.1(Human)