N-Acetylgalactosamine-6-Sulfatase/GALNS Overexpression Lysate (Native)


Western Blot: GALNS Overexpression Lysate (Adult Normal) [NBL1-10952] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GALNS.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

N-Acetylgalactosamine-6-Sulfatase/GALNS Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC208708 and based on accession number NM_000512. The protein contains a C-terminal DDK tag.
Homo sapiens galactosamine (N-acetyl)-6-sulfate sulfatase (GALNS), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
55.4 kDa

Lysate Details for N-Acetylgalactosamine-6-Sulfatase/GALNS

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for N-Acetylgalactosamine-6-Sulfatase/GALNS Overexpression Lysate (Native)

  • Chondroitinase
  • Chondroitinsulfatase
  • EC 3.1.6
  • EC
  • FLJ17434
  • FLJ42844
  • FLJ98217
  • GA6S
  • galactosamine (N-acetyl)-6-sulfate sulfatase
  • Galactose-6-Sulfate Sulfatase
  • GalNAc6S Sulfatase
  • GAS
  • MPS4A
  • NAcetylgalactosamine6Sulfatase
  • N-acetylgalactosamine-6-sulfatase
  • N-acetylgalactosamine-6-sulfate sulfatase


This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for N-Acetylgalactosamine-6-Sulfatase/GALNS Lysate (NBL1-10952) (0)

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Gene Symbol GALNS

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