Myosin 1C Antibody - BSA Free

Western Blot: Myosin 1C Antibody - BSA Free [Myosin 1C] - Western blot analysis of various lysates, using Myosin 1C Rabbit pAb at 1:1500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

Immunohistochemistry-Paraffin: Myosin 1C Antibody [NBP2-95224] - Paraffin-embedded mouse kidney using Myosin 1C .

Western Blot: Myosin 1C Antibody [NBP2-95224] - Paraffin-embedded rat kidney using Myosin 1C .

Immunoprecipitation: Myosin 1C Antibody - BSA Free [Myosin 1C] - Immunoprecipitation analysis of 300ug extracts of A549 cells using 3ug Myosin 1C Rabbit pAb . Western blot was performed from the immunoprecipitate using Myosin 1C Rabbit pAb at a dilition of 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Myosin 1C Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Myosin 1C Antibody - BSA Free (NBP2-95224) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 769-1028 of human MYO1C (NP_203693.3). ENAFFLDHVRTSFLLNLRRQLPQNVLDTSWPTPPPALREASELLRELCIKNMVWKYCRSISPEWKQQLQQKAVASEIFKGKKDNYPQSVPRLFISTRLGTDEISPRVLQALGSEPIQYAVPVVKYDRKGYKPRSRQLLLTPNAVVIVEDAKVKQRIDYANLTGISVSSLSDSLFVLHVQRADNKQKGDVVLQSDHVIETLTKTALSANRVNSININQGSITFAGGPGRDGTIDFTPGSELLITKAKNGHLAVVAPRLNSR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYO1C
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:50 - 1:200
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Myosin 1C Antibody - BSA Free

• FLJ23903
• MMIb
• MMI-beta
• Myosin I beta
• myosin IC
• myosin-I beta
• myosin-Ic
• myr2
• NMI
• nuclear myosin I

Background

Myosin 1C encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. The related gene myosin IE has been referred to as myosin IC in the literature, but it is a distinct locus on chromosome 19. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYO1C