MyoD Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: MyoD1 Antibody [NBP1-54153] - Detection of MYOD1 in RH30 cells.

Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - Staing of MYOD1 in RD cells with FITC (green). Nuclei were counterstained with DAPI (blue).

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MyoD Antibody - BSA Free Summary

• Immunogen: A partial recombinant protein made to an N-terminal region of the human MyoD1 protein (within residues 1-150). [Swiss-Prot# P15172]
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYOD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Western Blot 1:5000
• Application Notes: This MyoD1 antibody is useful for ICC/IF and Western blot, where a band is seen ~42 kDa.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28569745).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for MyoD Antibody - BSA Free

• bHLHc1
• bHLHc1BHLHC1
• Class C basic helix-loop-helix protein 1
• MYF3
• MYF3Myf-3
• MyoD
• MYOD1
• MYODmyoblast determination protein 1
• myogenic differentiation 1
• Myogenic factor 3myf-3
• PUM

Background

MyoD1 is a myogenic bHLH transcription factor which is implicated in muscle differentiation via activation of muscle-specific promoters and is considered a master regulator of skeletal myogenesis as its expression induces myogenic differentiation in myoblasts, fibroblasts, and certain other cell types. MyoD1 is expressed during muscle development and remains silent in most cell types, and is associated with modified histones targeted to repressed genes. MyoD1 primers have been designed to amplify DNA in exon 1 of human MyoD1 gene locus and these primer sets are supplied premixed with forward and reverse primers in 10 uM in TE buffer. MyoD1 primers are compatible with all real-time PCR reactions and each primer set has been test validated by ChIP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MyoD Antibody (NBP1-54153)(6)

Publications using NBP1-54153

• Horioka K, Tanaka H, Okaba K et al. Bioprotective role of platelet-derived microvesicles in hypothermia: Insight into the differential characteristics of peripheral and splenic platelets Thrombosis research 2023-03-01 [PMID: 36758284] (IHC-P, Mouse)
• Zhang Q, Vashisht A, O'Rourke J et al. The microprotein Minion controls cell fusion and muscle formation Nature Communications 2017-06-01 [PMID: 28569745] (ICC/IF, Mouse)
• Corbel S, Sampath S, Schmedt C, Zhang Q COMPOSITIONS, METHODS, AND THERAPEUTIC USES RELATED TO FUSOGENIC PROTEIN MINION United States Patent Application 20190263899 2019-08-29
• Yehezkely R, Zaffryar-Eilot S, Kaganovsky A et al. Intracellular Role for the Matrix-Modifying Enzyme Lox in Regulating Transcription Factor Subcellular Localization and Activity in Muscle Regeneration Developmental Cell 2020-05-18 [PMID: 32359406] (IHC-Fr, Mouse)
• Jin Z, Da W, Huang C et al. Synergistic effects of herbal compounds to promote osteoporotic fracture repair through upregulation of B-catenin signaling in skeletal muscle satellite cells Authorea 2022-01-01 (IHC-P, WB, Mouse)
  
  Details:
  Dilution used in WB 1:4000, in IHC 1:200
• Huang J, Jian X, Xu M et al. Muscle cytotoxicity and immuno-reactivity analysis of the porous carbon nanospheres fabricated by high temperature calcination Nanomedicine : nanotechnology, biology, and medicine 2022-11-23 [PMID: 36435365] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: MYOD1
• Entrez:
  • 4654(Human)
  • 17927(Mouse)
• Uniprot:
  • P15172()
  • P10085(Mouse)