MyoD Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated ...read more
Western Blot: MyoD1 Antibody [NBP1-54153] - Detection of MYOD1 in RH30 cells.
Immunocytochemistry/ Immunofluorescence: MyoD Antibody [NBP1-54153] - Staing of MYOD1 in RD cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

MyoD Antibody - BSA Free Summary

Immunogen
A partial recombinant protein made to an N-terminal region of the human MyoD1 protein (within residues 1-150). [Swiss-Prot# P15172]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYOD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Western Blot 1:5000
Application Notes
This MyoD1 antibody is useful for ICC/IF and Western blot, where a band is seen ~42 kDa.
Publications
Read Publications using
NBP1-54153 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28569745).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for MyoD Antibody - BSA Free

  • bHLHc1
  • bHLHc1BHLHC1
  • Class C basic helix-loop-helix protein 1
  • MYF3
  • MYF3Myf-3
  • MyoD
  • MYOD1
  • MYODmyoblast determination protein 1
  • myogenic differentiation 1
  • Myogenic factor 3myf-3
  • PUM

Background

MyoD1 is a myogenic bHLH transcription factor which is implicated in muscle differentiation via activation of muscle-specific promoters and is considered a master regulator of skeletal myogenesis as its expression induces myogenic differentiation in myoblasts, fibroblasts, and certain other cell types. MyoD1 is expressed during muscle development and remains silent in most cell types, and is associated with modified histones targeted to repressed genes. MyoD1 primers have been designed to amplify DNA in exon 1 of human MyoD1 gene locus and these primer sets are supplied premixed with forward and reverse primers in 10 uM in TE buffer. MyoD1 primers are compatible with all real-time PCR reactions and each primer set has been test validated by ChIP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MyoD Antibody (NBP1-54153)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, IHC-Fr.


Filter By Application
ICC/IF
(1)
IHC-Fr
(1)
All Applications
Filter By Species
Mouse
(2)
All Species

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Product General Protocols

View specific protocols for MyoD Antibody (NBP1-54153): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYOD1
Entrez
Uniprot