MyoD Antibody (SPM427) [DyLight 680]

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Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Clone
SPM427
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 680

Order Details

MyoD Antibody (SPM427) [DyLight 680] Summary

Immunogen
Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189.
Localization
Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Marker
Rhabdomyosarcoma Marker
Specificity
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYOD1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MyoD Antibody (SPM427) [DyLight 680]

  • bHLHc1
  • bHLHc1BHLHC1
  • Class C basic helix-loop-helix protein 1
  • MYF3
  • MYF3Myf-3
  • MyoD
  • MYOD1
  • MYODmyoblast determination protein 1
  • myogenic differentiation 1
  • Myogenic factor 3myf-3
  • PUM

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34772AF350
Alexa Fluor 405 NBP2-34772AF405
Alexa Fluor 488 NBP2-34772AF488
Alexa Fluor 532 NBP2-34772AF532
Alexa Fluor 594 NBP2-34772AF594
Alexa Fluor 647 NBP2-34772AF647
Alexa Fluor 700 NBP2-34772AF700
Alexa Fluor 750 NBP2-34772AF750
Allophycocyanin NBP2-34772APC
Biotin NBP2-34772B
DyLight 350 NBP2-34772UV
DyLight 405 NBP2-34772V
DyLight 488 NBP2-34772G
DyLight 550 NBP2-34772R
DyLight 594 NBP2-34772DL594
DyLight 650 NBP2-34772C
DyLight 680 NBP2-34772FR
DyLight 755 NBP2-34772IR
FITC NBP2-34772F
HRP NBP2-34772H
Janelia Fluor 549 NBP2-34772JF549
Janelia Fluor 646 NBP2-34772JF646
PE NBP2-34772PE
PerCP NBP2-34772PCP

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Bioinformatics Tool for MyoD Antibody (NBP2-34772FR)

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Bioinformatics

Gene Symbol MYOD1