Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) [NBP2-32882] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Antibody (SPM427).
Flow (Intracellular): MyoD Antibody (SPM427) [NBP2-32882] - An intracellular stain was performed on RD cells with MyoD Antibody [SPM427] NBP2-34772PE (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) [NBP2-32882] - Formalin-paraffin rhabdomyosarcoma stained with MyoD1 MAb (SPM427)
Flow (Intracellular): MyoD Antibody (SPM427) [NBP2-32882] - An intracellular stain was performed on RH-30 cells with MyoD Antibody (SPM427) NBP2-34772PE (blue) and a matched isotype control (orange). Cells were fixed ...read more
Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189.
Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.
Protein A or G purified
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Use in WB reported in scientific literature (PMID:34573307). Immunohistochemistry (Formalin-fixed). : 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP2-32882 in the following applications:
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