MyoD Antibody (SPM427)

Images

 
Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) [NBP2-32882] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Antibody (SPM427).
Flow (Intracellular): MyoD Antibody (SPM427) [NBP2-32882] - An intracellular stain was performed on RD cells with MyoD Antibody [SPM427] NBP2-34772PE (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) [NBP2-32882] - Formalin-paraffin rhabdomyosarcoma stained with MyoD1 MAb (SPM427)
Flow (Intracellular): MyoD Antibody (SPM427) [NBP2-32882] - An intracellular stain was performed on RH-30 cells with MyoD Antibody (SPM427) NBP2-34772PE (blue) and a matched isotype control (orange). Cells were fixed ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
Clone
SPM427
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

MyoD Antibody (SPM427) Summary

Immunogen
Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189.
Localization
Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Marker
Rhabdomyosarcoma Marker
Specificity
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYOD1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Western Blot
Application Notes
Use in WB reported in scientific literature (PMID:34573307). Immunohistochemistry (Formalin-fixed). : 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-32882 in the following applications:

  • IHC
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34772)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for MyoD Antibody (SPM427)

  • bHLHc1
  • bHLHc1BHLHC1
  • Class C basic helix-loop-helix protein 1
  • MYF3
  • MYF3Myf-3
  • MyoD
  • MYOD1
  • MYODmyoblast determination protein 1
  • myogenic differentiation 1
  • Myogenic factor 3myf-3
  • PUM

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MyoD Antibody (NBP2-32882)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYOD1
Uniprot