MyoD Antibody (SPM427) - Azide and BSA Free

Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) - Azide and BSA Free [NBP2-34772] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Antibody (SPM427).

Flow (Intracellular): MyoD Antibody (SPM427) - Azide and BSA Free [NBP2-34772] - Flow (Intracellular): MyoD Antibody (SPM427) [NBP2-32882] - An intracellular stain was performed on RD cells with MyoD Antibody [SPM427] NBP2-34772PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. Image using the PE Azide and BSA free format of this antibody.

Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) - Azide and BSA Free [NBP2-34772] - Formalin-paraffin rhabdomyosarcoma stained with MyoD1 MAb (SPM427)

Flow Cytometry: MyoD Antibody (SPM427) - Azide and BSA Free [NBP2-34772] - An intracellular stain was performed on RH-30 cells with MyoD Antibody (SPM427) NBP2-34772PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: Flow, Flow-IC, ICC/IF, IHC, IHC-P, CyTOF-ready
• Clone: SPM427
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

MyoD Antibody (SPM427) - Azide and BSA Free Summary

• Immunogen: Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189.
• Localization: Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
• Marker: Rhabdomyosarcoma Marker
• Specificity: Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYOD1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • CyTOF-ready
  • Flow (Intracellular)
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Notes

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A or G prepared in 10 mM PBS WITHOUT BSA.

Alternate Names for MyoD Antibody (SPM427) - Azide and BSA Free

• bHLHc1
• bHLHc1BHLHC1
• Class C basic helix-loop-helix protein 1
• MYF3
• MYF3Myf-3
• MyoD
• MYOD1
• MYODmyoblast determination protein 1
• myogenic differentiation 1
• Myogenic factor 3myf-3
• PUM

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MyoD Antibody (NBP2-34772)(1)

Publication using NBP2-34772

• Ocasio J, Babcock B, Malawsky D, et al. scRNA-seq in medulloblastoma shows cellular heterogeneity and lineage expansion support resistance to SHH inhibitor therapy Nat Commun Dec 20 2019 [PMID: 31863004] (IHC, Mouse)

Bioinformatics

• Gene Symbol: MYOD1