MyoD Antibody (MYOD1/3418R)

Immunohistochemistry-Paraffin: MyoD Antibody (MYOD1/3418R) [NBP3-07404] - Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD Recombinant Rabbit Monoclonal Antibody (MYOD1/3418R). HIER: Tris/EDTA pH9.0; 95C/45min. 1 Ab: 2ug/ml in PBS:30min. 2 Ab: HRP-Poly:30min. DAB:5min.

Immunohistochemistry-Paraffin: MyoD Antibody (MYOD1/3418R) [NBP3-07404] - Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD Recombinant Rabbit Monoclonal Antibody (MYOD1/3418R). HIER: Tris/EDTA pH9.0; 95C/45min. 1 Ab: 2ug/ml in PBS:30min. 2 Ab: HRP-Poly:30min. DAB:5min

• Reactivity: Hu
• Applications: IHC
• Clone: MYOD1/3418R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MyoD Antibody (MYOD1/3418R) Summary

• Description: 200ug/ml of antibody purified by Protein A Column. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08543)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa1-100) of human MyoD protein (exact sequence is proprietary) (Uniprot: P15172 )
• Localization: Nuclear
• Marker: Rhabdomyosarcoma Marker
• Specificity: MyoD1, one of the MyoD family of myogenic helix-loop-helix transcription factors, combined with myogenin, plays a role in coordinating the myogenic differentiation pathway from the determination of mesodermal precursors into myoblasts, the differentiation of myoblasts into myotubes, and finally the maturation of myotubes into skeletal myofibers. Normal mature skeletal muscle does not express MyoD1 protein. MyoD1 is expressed in myoblasts before differentiation while myogenin has post-differentiation functions. Anti-MyoD1 immunostaining identifies cells committed to myogenesis in their earliest phase, thus, it is a better biomarker for less differentiated Rhabdomyosarcomas (RMS). RMS are the most frequent malignant soft tissue neoplasms of childhood. While better differentiated RMS have cross-striations or rhabdomyoblasts that allow for a confident morphologic diagnosis, less differentiated RMS resemble other small blue round-cell tumors. Studies suggest, anti-MyoD1 may be used together with anti-Myogenin and anti-Desmin as a panel of markers since any RMS is virtually never negative for all three markers simultaneously.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MYOD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for MyoD Antibody (MYOD1/3418R)

• bHLHc1
• bHLHc1BHLHC1
• Class C basic helix-loop-helix protein 1
• MYF3
• MYF3Myf-3
• MyoD
• MYOD1
• MYODmyoblast determination protein 1
• myogenic differentiation 1
• Myogenic factor 3myf-3
• PUM

Background

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYOD1
• Uniprot:
  • P15172()
  • P15172()